Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum

Masayuki Sakurai; Takashi Ohtsuki; Tsutomu Suzuki; Kimitsuna Watanabe

doi:10.1016/j.febslet.2005.04.009

Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum

Masayuki Sakurai, Takashi Ohtsuki, Tsutomu Suzuki, Kimitsuna Watanabe

研究成果 › 査読

23 被引用数 (Scopus)

抄録

To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm⁵U) was identified at the wobble positions of tRNA ^Lys, tRNA^Glu and tRNA^Gln, while 5-carboxymethylaminomethyl-2-thiouridine (cmnm⁵s²U) was present in tRNAUAALeuandtRNATrp. In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm⁵s²U is used in tRNAUAALeuandtRNATrp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.

本文言語	English
ページ（範囲）	2767-2772
ページ数	6
ジャーナル	FEBS Letters
巻	579
号	13
DOI	https://doi.org/10.1016/j.febslet.2005.04.009
出版ステータス	Published - 5月 23 2005

ASJC Scopus subject areas

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/j.febslet.2005.04.009

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title = "Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum",

abstract = "To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm5U) was identified at the wobble positions of tRNA Lys, tRNAGlu and tRNAGln, while 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) was present in tRNAUAALeuandtRNATrp. In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm5s2U is used in tRNAUAALeuandtRNATrp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.",

keywords = "2-Thiouridine, Mitochondrial tRNA, Modified nucleoside, Nematode, T-armless tRNA, Wobble position",

author = "Masayuki Sakurai and Takashi Ohtsuki and Tsutomu Suzuki and Kimitsuna Watanabe",

note = "Funding Information: This work was supported by Grants-in-Aid from the Ministry of Culture, Sports, Science and Technology, Japan to K.W. and T.O. We thank Dr. Y. Watanabe for materials and valuable discussions, and Dr. K. Kita, Dr. N. Shigi and Mr. Y. Yamamoto for materials. We are grateful to Dr. Takeo Suzuki and Mr. Y. Ikeuchi for their technical assistance on tRNA isolation and mass spectrometry. ",

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AU - Ohtsuki, Takashi

AU - Suzuki, Tsutomu

AU - Watanabe, Kimitsuna

N1 - Funding Information: This work was supported by Grants-in-Aid from the Ministry of Culture, Sports, Science and Technology, Japan to K.W. and T.O. We thank Dr. Y. Watanabe for materials and valuable discussions, and Dr. K. Kita, Dr. N. Shigi and Mr. Y. Yamamoto for materials. We are grateful to Dr. Takeo Suzuki and Mr. Y. Ikeuchi for their technical assistance on tRNA isolation and mass spectrometry.

PY - 2005/5/23

Y1 - 2005/5/23

N2 - To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm5U) was identified at the wobble positions of tRNA Lys, tRNAGlu and tRNAGln, while 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) was present in tRNAUAALeuandtRNATrp. In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm5s2U is used in tRNAUAALeuandtRNATrp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.

AB - To understand the decoding property of nematode mitochondrial tRNAs with unusual secondary structures, post-transcriptional modifications at wobble positions of Ascaris suum mitochondrial tRNAs corresponding to two-codon families ending with a purine were analyzed. 5-Carboxymethylaminomethyluridine (cmnm5U) was identified at the wobble positions of tRNA Lys, tRNAGlu and tRNAGln, while 5-carboxymethylaminomethyl-2-thiouridine (cmnm5s2U) was present in tRNAUAALeuandtRNATrp. In most bacterial and mitochondrial tRNAs, the 2-thiouridine derivative is present in tRNAs for Lys, Glu and Gln. These is no report that cmnm5s2U is used in tRNAUAALeuandtRNATrp. The unusual usage of wobble modifications might assist decoding of nematode mitochondrial mRNAs.

KW - 2-Thiouridine

KW - Mitochondrial tRNA

KW - Modified nucleoside

KW - Nematode

KW - T-armless tRNA

KW - Wobble position

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Unusual usage of wobble modifications in mitochondrial tRNAs of the nematode Ascaris suum

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