@article{4d27cc8abf284072a1298a27087322cf,
title = "The primary structure of the Cl--Translocating ATPase, b subunit of Acetabularia acetabulum, which belongs to the F-type ATpase family",
abstract = "The genes possibly encoding the b subunit (50 kDa) of the Cl - translocating ATPase of Acetabularia acetabulum were cloned from total RNA and from poly(A)1 RNA and sequenced. The deduced amino acid sequence of the open reading frame consisted of 478 amino acids and showed high similarity to the β subunit of chloroplast F1-ATPase. Gene fragments encoding the putative β subunit of chloroplast F1- (273 bp) and mitochondrial F1- ATPases (332 bp) were also cloned from A. acetabulum and sequenced, respectively. The deduced amino acid sequence of the chloroplast F1-ATPase showed 92.5% identity to the primary structure of the b subunit of the Cl - translocating ATPase, while the nucleotide sequences were 79.9% identical. The deduced amino acid sequence of the latter was 77.3% identical to that of the b subunit of the Cl-translocating ATPase and the nucleotide sequences were 67.5% identical. By Northern analysis, these three β-like genes were demonstrated to be transcribed with different sizes of RNA species. A putative chloroplast F1-β fragment also hybridized with chloroplast DNA isolated from the organism.",
keywords = "ATPase, Acetabularia acetabulum, Cl -translocation, F-type, b subunit, cloning",
author = "Chie Moritani and Toshitaka Ohhashi and Hiromi Kadowaki and Mitsuo Tagaya and Toshio Fukui and Friedrich Lottspeich and Dieter Oesterhelt and Mikiko Ikeda",
note = "Funding Information: We are grateful to Mrs. U. Shimanko of the Max-Planck-Institute of Biochemistry for the synthesis of oligonucleotides required as PCR and sequencing primers. Thanks are extended to Dr. K. Inagaki of Okayama University for amino acid sequencing. This work was partly supported by grants under the Monbusho International Scien-ti{\textregistered}c Research Program (02044101 and 04044123), by a Grant-in-Aid for Scienti{\textregistered}c Research Program (C) (04671352) from the Ministry of Education, Science and Culture, by a grant from Takeda Science Promotion Society, and by a grant from Hayashi Memorial Foundation for Female Natural Scientists. We are also grateful to Professor Dr. S. Ohmori of Okayama University for supporting this work and to Dr. J. A. Shiozawa of the Max-Planck-Institute of Biochemistry for reading the manuscript and improving the English.",
year = "1997",
month = mar,
day = "1",
doi = "10.1006/abbi.1996.9845",
language = "English",
volume = "339",
pages = "115--124",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "1",
}