TY - JOUR
T1 - Tethering assays for COPI vesicles mediated by golgins
AU - Satoh, Ayano
AU - Malsam, Jörg
AU - Warren, Graham
N1 - Funding Information:
We thank all members of the Warren, Mellman, and Toomre laboratories for helpful comments and discussions, and Marino Zerial for generous provision of purified EEA1. This work was supported by the NIH and the Ludwig Institute for Cancer Research. A.S. was supported by the American Heart Association.
PY - 2005
Y1 - 2005
N2 - A method is described that allows the attachment of COPI vesicles and Golgi membranes to glass slides that can then be analyzed using electron microscopy (EM) and immuno-EM methods. Subpopulations of COPI vesicles can be bound selectively using recombinant golgins. Alternatively, COPI vesicles can be attached to prebound Golgi membranes. Marking these vesicles selectively with biotin allows their site of attachment to be identified.
AB - A method is described that allows the attachment of COPI vesicles and Golgi membranes to glass slides that can then be analyzed using electron microscopy (EM) and immuno-EM methods. Subpopulations of COPI vesicles can be bound selectively using recombinant golgins. Alternatively, COPI vesicles can be attached to prebound Golgi membranes. Marking these vesicles selectively with biotin allows their site of attachment to be identified.
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U2 - 10.1016/S0076-6879(05)04013-9
DO - 10.1016/S0076-6879(05)04013-9
M3 - Review article
C2 - 16413264
AN - SCOPUS:30544440210
SN - 0076-6879
VL - 404
SP - 125
EP - 134
JO - Methods in Enzymology
JF - Methods in Enzymology
M1 - 13
ER -