Structural Analyses of Sugar Chains from Ricin A-Chain Variant

Yoshinobu Kimura; Hironobu Kusuoku; Mikiro Tada; Shigeaki Takagi; Gunki Funatsu

doi:10.1080/00021369.1990.10869890

Structural Analyses of Sugar Chains from Ricin A-Chain Variant

Yoshinobu Kimura, Hironobu Kusuoku, Mikiro Tada, Shigeaki Takagi, Gunki Funatsu

研究成果 › 査読

16 被引用数 (Scopus)

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Two glycopeptides were isolated from a tryptic digest of ricin A-chain variant by gel filtration and reversed-phase HPLC. Their amino acid compositions and sequences showed that Asn-10 and Asn-236 are glycosylated. The sugar chains were liberated from these glycopeptides by hydrazynolysis, N-acetylated, and pyridylaminated. Component analysis and comparison of elution positions of the purified pyridylamino (PA-)sugar chains with those of authentic PA-sugar chains from the major ricin A-chain by reversed-phase HPLC and size fractionation HPLC suggested that both sugar chains linked to Asn-10 and Asn-236 of the ricin A-chain variant are M3FX, identical to that linked to Asn-10 of the major ricin A-chain.

本文言語	English
ページ（範囲）	157-162
ページ数	6
ジャーナル	Agricultural and Biological Chemistry
巻	54
号	1
DOI	https://doi.org/10.1080/00021369.1990.10869890
出版ステータス	Published - 1990

ASJC Scopus subject areas

農業および生物科学（全般）
生化学、遺伝学、分子生物学（全般）

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10.1080/00021369.1990.10869890

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abstract = "Two glycopeptides were isolated from a tryptic digest of ricin A-chain variant by gel filtration and reversed-phase HPLC. Their amino acid compositions and sequences showed that Asn-10 and Asn-236 are glycosylated. The sugar chains were liberated from these glycopeptides by hydrazynolysis, N-acetylated, and pyridylaminated. Component analysis and comparison of elution positions of the purified pyridylamino (PA-)sugar chains with those of authentic PA-sugar chains from the major ricin A-chain by reversed-phase HPLC and size fractionation HPLC suggested that both sugar chains linked to Asn-10 and Asn-236 of the ricin A-chain variant are M3FX, identical to that linked to Asn-10 of the major ricin A-chain.",

author = "Yoshinobu Kimura and Hironobu Kusuoku and Mikiro Tada and Shigeaki Takagi and Gunki Funatsu",

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T1 - Structural Analyses of Sugar Chains from Ricin A-Chain Variant

AU - Kimura, Yoshinobu

AU - Kusuoku, Hironobu

AU - Tada, Mikiro

AU - Takagi, Shigeaki

AU - Funatsu, Gunki

PY - 1990

Y1 - 1990

N2 - Two glycopeptides were isolated from a tryptic digest of ricin A-chain variant by gel filtration and reversed-phase HPLC. Their amino acid compositions and sequences showed that Asn-10 and Asn-236 are glycosylated. The sugar chains were liberated from these glycopeptides by hydrazynolysis, N-acetylated, and pyridylaminated. Component analysis and comparison of elution positions of the purified pyridylamino (PA-)sugar chains with those of authentic PA-sugar chains from the major ricin A-chain by reversed-phase HPLC and size fractionation HPLC suggested that both sugar chains linked to Asn-10 and Asn-236 of the ricin A-chain variant are M3FX, identical to that linked to Asn-10 of the major ricin A-chain.

AB - Two glycopeptides were isolated from a tryptic digest of ricin A-chain variant by gel filtration and reversed-phase HPLC. Their amino acid compositions and sequences showed that Asn-10 and Asn-236 are glycosylated. The sugar chains were liberated from these glycopeptides by hydrazynolysis, N-acetylated, and pyridylaminated. Component analysis and comparison of elution positions of the purified pyridylamino (PA-)sugar chains with those of authentic PA-sugar chains from the major ricin A-chain by reversed-phase HPLC and size fractionation HPLC suggested that both sugar chains linked to Asn-10 and Asn-236 of the ricin A-chain variant are M3FX, identical to that linked to Asn-10 of the major ricin A-chain.

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Structural Analyses of Sugar Chains from Ricin A-Chain Variant

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