@article{22b0b6fd6052428fa80c31fad7c5c732,
title = "Stress induced factor 2 regulates arabidopsis stomatal immunity through phosphorylation of the anion channel SLAC1",
abstract = "Upon recognition of microbes, pattern recognition receptors (PRRs) activate pattern-triggered immunity. FLAGELLIN SENSING2 (FLS2) and BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE1 (BAK1) form a typical PRR complex that senses bacteria. Here, we report that the kinase activity of the malectin-like receptor-like kinase STRESS INDUCED FACTOR 2 (SIF2) is critical for Arabidopsis (Arabidopsis thaliana) resistance to bacteria by regulating stomatal immunity. SIF2 physically associates with the FLS2-BAK1 PRR complex and interacts with and phosphorylates the guard cell SLOW ANION CHANNEL1 (SLAC1), which is necessary for abscisic acid (ABA)-mediated stomatal closure. SIF2 is also required for the activation of ABA-induced S-type anion currents in Arabidopsis protoplasts, and SIF2 is sufficient to activate SLAC1 anion channels in Xenopus oocytes. SIF2-mediated activation of SLAC1 depends on specific phosphorylation of Ser 65. This work reveals that SIF2 functions between the FLS2-BAK1 initial immunity receptor complex and the final actuator SLAC1 in stomatal immunity.",
author = "Ching Chan and Dario Panzeri and Eiji Okuma and Kadri T{\~o}ldsepp and Wang, {Ya Yun} and Louh, {Guan Yu} and Chin, {Tzu Chuan} and Yeh, {Yu Hung} and Yeh, {Hung Ling} and Shweta Yekondi and Huang, {You Huei} and Huang, {Tai Yuan} and Chiou, {Tzyy Jen} and Yoshiyuki Murata and Hannes Kollist and Laurent Zimmerli",
note = "Funding Information: We thank the ABRC for providing seeds and Barbara N. Kunkel and Chao-Ying Chen for bacteria and B. cinerea, respectively. We also thank the Technology Commons (TechComm), College of Life Science, National Taiwan University for providing RT-qPCR equipment. We appreciate the help of Yi-Fang Tsay (Academia Sinica, Taiwan) in providing technical and instrumental support for the oocyte patch clamp. This work was supported by the Ministry of Science and Technology of Taiwan (grants 99-2628-B-002-053-MY3, 102-2628-B-002-011-MY3, and 105-2311-B-002-032-MY3 to L.Z.) and the National Taiwan University, (Frontier and Innovative Research grant 99R70436 to L.Z.). Support was also provided by the Estonian Ministry of Science and Education (grant PRG433 to H.K.) and by the Center of Excellence in Molecular Cell Engineering (European Regional Fund). Publisher Copyright: {\~a} 2020 ASPB.",
year = "2020",
month = jul,
doi = "10.1105/tpc.19.00578",
language = "English",
volume = "32",
pages = "2216--2236",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "7",
}