Screening of edible Japanese plants for suppressive effects on phorbol ester-induced superoxide generation in differentiated HL-60 cells and AS52 cells

Ha Won Kim, Akira Murakami, Yoshimasa Nakamura, Hajime Ohigashi

研究成果査読

30 被引用数 (Scopus)

抄録

Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O2-) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O2- in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O2- generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O2- generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.

本文言語English
ページ(範囲)7-16
ページ数10
ジャーナルCancer Letters
176
1
DOI
出版ステータスPublished - 2月 8 2002
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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