TY - JOUR
T1 - Properties of the na+/h+ antiporter in Vibrio parahaemolyticus1
AU - Kuroda, Teruo
AU - Shimamoto, Toshi
AU - Inaba, Kei
AU - Kayahara, Takashi
AU - Tsuda, Masaaki
AU - Tsuchiya, Tomofusa
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1994/6
Y1 - 1994/6
N2 - The properties of the Na+/H+ antiporter in Vibrio parahaemolyticus, a slightly halophilic bacterium, were investigated using everted membrane vesicles. It appears that at least two Na+/H+ antiporters are present, one that is pH-dependent and one that is pH-independent. These two antiporters appear to correspond to the NhaA and NhaB antiporters of Escherichia coli, respectively. It seems that amiloride strongly inhibits the pH-dependent ntiporter. Na+ is the best substrate for both of the two V. parahaemolyticus antiporters. Li+ is a poorer substrate and K+ is not a substrate. No K++H+ antiport activity was detected in membrane vesicles of this organism. The Na+(Li+)/H+ antiport activity greatly increased with an increase in pH of the assay medium. pH did not affect the Km value of the Na+/H+ antiport, but it did increase the Vmax.
AB - The properties of the Na+/H+ antiporter in Vibrio parahaemolyticus, a slightly halophilic bacterium, were investigated using everted membrane vesicles. It appears that at least two Na+/H+ antiporters are present, one that is pH-dependent and one that is pH-independent. These two antiporters appear to correspond to the NhaA and NhaB antiporters of Escherichia coli, respectively. It seems that amiloride strongly inhibits the pH-dependent ntiporter. Na+ is the best substrate for both of the two V. parahaemolyticus antiporters. Li+ is a poorer substrate and K+ is not a substrate. No K++H+ antiport activity was detected in membrane vesicles of this organism. The Na+(Li+)/H+ antiport activity greatly increased with an increase in pH of the assay medium. pH did not affect the Km value of the Na+/H+ antiport, but it did increase the Vmax.
KW - PH-dependence
KW - Properties
KW - Substrate specificity
KW - Vibrio parahaemolyticus
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U2 - 10.1093/oxfordjournals.jbchem.a124473
DO - 10.1093/oxfordjournals.jbchem.a124473
M3 - Article
C2 - 7982898
AN - SCOPUS:0028318263
SN - 0021-924X
VL - 115
SP - 1162
EP - 1165
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 6
ER -