Preparation of hydroxyapatite-nanocrystal-coated stainless steel, and its cell interaction

Masahiro Okada, Miwa Masuda, Ryoichi Tanaka, Kunio Miyatake, Daisuke Kuroda, Tsutomu Furuzono


8 被引用数 (Scopus)


Calcined nanocrystals of hydroxyapatite (HAp) having spherical or rod-shaped morphologies were coated through covalent linkage on a type 316L stainless steel substrate, which was chemically modified by the graft polymerization of γ-methacryloxypropyl triethoxysilane (MPTS) at 70-110°C. The grafting of poly(MPTS) on the substrate was confirmed by X-ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR). In order to coat the substrate with the HAp crystals through covalent linkage, the reaction between the alkoxysilyl groups in the poly(MPTS) grafted on the substrate and the OH groups on the HAp crystals was conducted at 80°C. The poly(MPTS)-grafted substrate was strongly coated with the HAp nanocrystals, although the HAp crystals adsorbed physically on the original substrate without poly(MPTS) grafting were removed by ultrasonic treatment. Human umbilical vein endothelial cells (HUVEC) adhered in larger numbers on the HAp-coated stainless steel substrate as compared with the original substrate after 24 h of initial incubation. The number of HUVEC adhered on the rod-shaped HAp-coated substrate was not significantly different from that on the spherical HAp-coated substrate under the present conditions.

ジャーナルJournal of Biomedical Materials Research - Part A
出版ステータスPublished - 9月 1 2008

ASJC Scopus subject areas

  • セラミックおよび複合材料
  • 生体材料
  • 生体医工学
  • 金属および合金


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