pp60^c-src expression in rat spermatogenesis

A fractionation of the membrane extract of rat testes revealed the existence of pp60^c-src kinase activity. The expression of pp60^c-src was examined in the developing rat testes. The immunecomplex kinase assay using a monoclonal antibody specific to pp60^c-src(mAb327) showed that the expression of pp60^c-src kinase activity increased during the development of rat testes and declined in the adult. The increase in pp60^c-src kinase activity observed during the development of rat testes was accompanied with an increase in the amount of pp60^c-src protein. The peak period in the increase of pp60^c-src kinase activity well coincided with the timing, when the spermatogenesis by meiosis just began. The immunohistochemical staining of pp60^c-src in rat testes demonstrated that pp60^c-src is most abundantly expressed in the spermatids which are the spermatogenic cells in the post-meiotic phase of the spermatogenesis. These findings strongly suggest that pp60^c-src is a developmentally regulated gene product which is involved in rat spermatogenesis.