TY - JOUR
T1 - Osteoblast Response of Additively Manufactured Zirconia and Alumina-Toughened Zirconia
AU - Nakai, Hiroto
AU - Inokoshi, Masanao
AU - Nozaki, Kosuke
AU - Yoshihara, Kumiko
AU - Matsukawa, Akihiro
AU - Nagaoka, Noriyuki
AU - Tonprasong, Watcharapong
AU - Minakuchi, Shunsuke
N1 - Funding Information:
This research was funded by a JSPS Grant-in-Aid for Scientific Research C (Grant Number JP19K10241), Iketani Science and Technology Foundation in 2022 (Grant Number 0341114-A), and JST SPRING (Grant Number JPMJSP2120).
Publisher Copyright:
© 2022 by the authors.
PY - 2022/12
Y1 - 2022/12
N2 - Zirconia ceramics have been widely used in dentistry. Herein, we assess the surface morphology, surface texture, and osteoblast response of additively manufactured zirconia and alumina-toughened zirconia (ATZ) in comparison with titanium. The surface roughness, contact angle, and surface microstructure of titanium sandblasted with large-grit alumina and subsequently acid-etched using 18% HCl and 49% H2SO4 (SLA-titanium), uniaxially pressed zirconia (UP zirconia), additively manufactured zirconia (AM zirconia), and additively manufactured ATZ (AM ATZ) were investigated. Moreover, the cell viability, alkaline phosphatase (ALP) activity, and gene expression of type I collagen on these materials were evaluated. The data were statistically analyzed using one-way ANOVA with Tukey’s post hoc test. SLA-titanium showed the highest surface roughness and contact angle. The other three materials showed comparable surface roughness and contact angles. Micro- and nanoroughness were observed on the surface of SLA-titanium. UP zirconia and AM zirconia had similar surface morphologies. The cell viability, ALP activity, and gene expression of type I collagen on AM zirconia were comparable to or better than those on SLA-titanium. Our results indicate that AM zirconia is a promising material for zirconia dental implants.
AB - Zirconia ceramics have been widely used in dentistry. Herein, we assess the surface morphology, surface texture, and osteoblast response of additively manufactured zirconia and alumina-toughened zirconia (ATZ) in comparison with titanium. The surface roughness, contact angle, and surface microstructure of titanium sandblasted with large-grit alumina and subsequently acid-etched using 18% HCl and 49% H2SO4 (SLA-titanium), uniaxially pressed zirconia (UP zirconia), additively manufactured zirconia (AM zirconia), and additively manufactured ATZ (AM ATZ) were investigated. Moreover, the cell viability, alkaline phosphatase (ALP) activity, and gene expression of type I collagen on these materials were evaluated. The data were statistically analyzed using one-way ANOVA with Tukey’s post hoc test. SLA-titanium showed the highest surface roughness and contact angle. The other three materials showed comparable surface roughness and contact angles. Micro- and nanoroughness were observed on the surface of SLA-titanium. UP zirconia and AM zirconia had similar surface morphologies. The cell viability, ALP activity, and gene expression of type I collagen on AM zirconia were comparable to or better than those on SLA-titanium. Our results indicate that AM zirconia is a promising material for zirconia dental implants.
KW - additive manufacturing
KW - alumina-toughened zirconia
KW - cell viability
KW - osteogenic ability
KW - zirconia
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U2 - 10.3390/ma15238685
DO - 10.3390/ma15238685
M3 - Article
AN - SCOPUS:85143836637
SN - 1996-1944
VL - 15
JO - Materials
JF - Materials
IS - 23
M1 - 8685
ER -