TY - JOUR
T1 - N-Linked Sugar Chains of 350-kDa Royal Jelly Glycoprotein
AU - Kimura, Yoshinobu
AU - Washino, Noriyuki
AU - Yonekura, Masami
PY - 1995
Y1 - 1995
N2 - The structures of N-linked sugar chains from 350 kDa royal jelly glycoprotein (RJG), which stimulates the proliferation of human monocytes, have been analyzed. The structural analyses of the N-linked sugar chains released by hydrazinolysis were done by two-dimensional sugar mapping of the corresponding pyridylamino derivatives and 500MHz 1H-NMR. All four structures were found to fall into the category of oligomannose-type sugar chains as follows; Manα 1→6 (Manα 1→3)Manα 1→6 (Manα 1→3) Manβ 1→4 GlcNAcβ 1→4 GlcNAc, Manα 1→2 Manα 1→6 (Manα 1→3) Manα 1→6 (Manα 1→2 Manα 1→3) Manβ 1 →4 GlcNAcβ 1 →4 GlcNAc, Manα 1→2Manα 1→6 (Manα 1→3) Manα 1→6 (Manα 1 →2 Manα 1→2Manα 1→3) Manβ 1→4 GlcNAcβ 1→4 GlcNAc, and Manα 1→2 Manα 1→6(Manα 1→2 Manα 1→3) Manα 1→6 (Manα 1→2 Manα 1→2 Manα 1→3) Manβ 1→4 GlcNAcβ 1→4 GlcNAc.
AB - The structures of N-linked sugar chains from 350 kDa royal jelly glycoprotein (RJG), which stimulates the proliferation of human monocytes, have been analyzed. The structural analyses of the N-linked sugar chains released by hydrazinolysis were done by two-dimensional sugar mapping of the corresponding pyridylamino derivatives and 500MHz 1H-NMR. All four structures were found to fall into the category of oligomannose-type sugar chains as follows; Manα 1→6 (Manα 1→3)Manα 1→6 (Manα 1→3) Manβ 1→4 GlcNAcβ 1→4 GlcNAc, Manα 1→2 Manα 1→6 (Manα 1→3) Manα 1→6 (Manα 1→2 Manα 1→3) Manβ 1 →4 GlcNAcβ 1 →4 GlcNAc, Manα 1→2Manα 1→6 (Manα 1→3) Manα 1→6 (Manα 1 →2 Manα 1→2Manα 1→3) Manβ 1→4 GlcNAcβ 1→4 GlcNAc, and Manα 1→2 Manα 1→6(Manα 1→2 Manα 1→3) Manα 1→6 (Manα 1→2 Manα 1→2 Manα 1→3) Manβ 1→4 GlcNAcβ 1→4 GlcNAc.
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U2 - 10.1271/bbb.59.507
DO - 10.1271/bbb.59.507
M3 - Article
C2 - 7766191
AN - SCOPUS:0029269727
SN - 0916-8451
VL - 59
SP - 507
EP - 509
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 3
ER -