Objectives The aim of this study was to evaluate enamel demineralization at composite restoration margins caused by cariogenic biofilm using swept-source optical coherence tomography (SS-OCT). Methods Sixty round-shaped cavities were prepared on the mid-buccal enamel surface of extracted human molars. The cavities were restored with Estelite Flow Quick flowable composite using either Clearfil SE Bond or Clearfil Tri-S Bond ND bonding agents. Streptococcus mutans suspension was applied to form a cariogenic biofilm on the surface. After 1, 2, or 3 weeks of incubation (n = 10), the biofilm was removed to observe the carious demineralization at the cavosurface margins using SS-OCT. The gap along the enamel–composite interface was recorded on each adhesive system. Confirmatory direct observation was accomplished at the same location using confocal laser scanning microscope. Results The demineralized enamel around the restorations was observed as a zone of intensified brightness in SS-OCT. The demineralized lesion on the cervical enamel was significantly deeper than that on the occlusal enamel (p < 0.05). However, the extension of enamel demineralization at the enamel–composite interface was significantly deeper at the occlusal wall than the cervical wall (p < 0.05). The extension in Tri-S Bond ND group was significantly deeper than in SE Bond group (p < 0.05). A significant increase in gap formation was found after the extension of demineralization compared with the baseline. Significance The carious demineralization around composite restorations were observed as a bright zone in SS-OCT during the process of bacterial demineralization. SS-OCT appears to be a promising modality for the detection of caries adjacent to an existing restoration.
|出版ステータス||Published - 9月 1 2016|
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