Isolation and characterization of the nuclei from Saccharomyces cerevisiae

Kazuo Kamimura; Eiko Tsuchiya; Tokichi Miyakawa; Sakuzo Fuki; Aiko Hirata

doi:10.1007/BF01642394

Isolation and characterization of the nuclei from Saccharomyces cerevisiae

Kazuo Kamimura, Eiko Tsuchiya, Tokichi Miyakawa, Sakuzo Fuki, Aiko Hirata

研究成果 › 査読

20 被引用数 (Scopus)

抄録

We isolated highly intact nuclei from cells of a haploid strain of Saccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.

本文言語	English
ページ（範囲）	175-180
ページ数	6
ジャーナル	Current Microbiology
巻	6
号	3
DOI	https://doi.org/10.1007/BF01642394
出版ステータス	Published - 5月 1981
外部発表	はい

ASJC Scopus subject areas

微生物学
応用微生物学とバイオテクノロジー

文献へのアクセス

10.1007/BF01642394

他のファイルとリンク

引用スタイル

@article{c4fd6607ef7148faa05d491968049096,

title = "Isolation and characterization of the nuclei from Saccharomyces cerevisiae",

abstract = "We isolated highly intact nuclei from cells of a haploid strain of Saccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.",

author = "Kazuo Kamimura and Eiko Tsuchiya and Tokichi Miyakawa and Sakuzo Fuki and Aiko Hirata",

year = "1981",

month = may,

doi = "10.1007/BF01642394",

language = "English",

volume = "6",

pages = "175--180",

journal = "Current Microbiology",

issn = "0343-8651",

publisher = "Springer New York",

number = "3",

}

TY - JOUR

T1 - Isolation and characterization of the nuclei from Saccharomyces cerevisiae

AU - Kamimura, Kazuo

AU - Tsuchiya, Eiko

AU - Miyakawa, Tokichi

AU - Fuki, Sakuzo

AU - Hirata, Aiko

PY - 1981/5

Y1 - 1981/5

N2 - We isolated highly intact nuclei from cells of a haploid strain of Saccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.

AB - We isolated highly intact nuclei from cells of a haploid strain of Saccharomyces cerevisiae. The isolated nuclei were spherical, maintained a double membrane with typical nuclear pores, and were free from mitochondrial-, vacuolar-, and cytoplasmic-marker enzymes. The nuclei could synthesize both DNA and RNA and could phosphorylate nuclear proteins in vitro. These biochemical activities were greatly affected by the osmotic treatment of the nuclei.

UR - http://www.scopus.com/inward/record.url?scp=0019814904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019814904&partnerID=8YFLogxK

U2 - 10.1007/BF01642394

DO - 10.1007/BF01642394

M3 - Article

AN - SCOPUS:0019814904

SN - 0343-8651

VL - 6

SP - 175

EP - 180

JO - Current Microbiology

JF - Current Microbiology

IS - 3

ER -

Isolation and characterization of the nuclei from Saccharomyces cerevisiae

抄録

ASJC Scopus subject areas

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル