TY - JOUR
T1 - Inhibition of placental ornithine decarboxylase by DL-α-difluoro-methyl ornithine causes fetal growth restriction in rat
AU - Ishida, Makoto
AU - Hiramatsu, Yuji
AU - Masuyama, Hisashi
AU - Mizutani, Yasushi
AU - Kudo, Takafumi
N1 - Funding Information:
Supported in part by a Grant-in-Aid for Scientific Research ( C ) number 08671895 from the Ministry of Education, Culture, Sports and Technology in Japan.
PY - 2002/2/8
Y1 - 2002/2/8
N2 - The roles of polyamines in intrauterine growth restriction (IUGR) is studied. The DL-α-difluoromethyl ornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC) which is a rate limiting enzyme of polyamine synthesis was administrated to pregnant rats so that we obtained rat fetuses with IUGR. The changes of maternal nutrition, damage of the placenta, and the direct effect of DFMO on the fetus were examined in this IUGR model. Administration of DFMO did not induced changes of maternal nutrition except for triglyceride and the fetal metabolic state. But the placental weight, ODC activity, and DNA in the placenta were decreased significantly. The ODC activity in the total placenta decreased to less than 10% of that of the control. Depression of ODC activity in the placenta may be the major cause of IUGR induced by DFMO administration, and polyamines play important roles to carry pregnancy.
AB - The roles of polyamines in intrauterine growth restriction (IUGR) is studied. The DL-α-difluoromethyl ornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC) which is a rate limiting enzyme of polyamine synthesis was administrated to pregnant rats so that we obtained rat fetuses with IUGR. The changes of maternal nutrition, damage of the placenta, and the direct effect of DFMO on the fetus were examined in this IUGR model. Administration of DFMO did not induced changes of maternal nutrition except for triglyceride and the fetal metabolic state. But the placental weight, ODC activity, and DNA in the placenta were decreased significantly. The ODC activity in the total placenta decreased to less than 10% of that of the control. Depression of ODC activity in the placenta may be the major cause of IUGR induced by DFMO administration, and polyamines play important roles to carry pregnancy.
KW - DL-α-difluoromethyl ornithine
KW - Intrauterine growth restriction
KW - Ornithine decarboxylase
KW - Rat
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U2 - 10.1016/S0024-3205(01)01510-7
DO - 10.1016/S0024-3205(01)01510-7
M3 - Article
C2 - 11883715
AN - SCOPUS:0037040036
SN - 0024-3205
VL - 70
SP - 1395
EP - 1405
JO - Life Sciences
JF - Life Sciences
IS - 12
ER -