TY - JOUR
T1 - In vivo tissue response of endodontic bio-ceramic materials
AU - Romano, Teresita
AU - Jammal, María Victoria
AU - Nakano, Keisuke
AU - Rusco, Ana García
AU - Fassi, Jorge Olmos
AU - Kozuszko, Silvia
AU - Takabatake, Kiyofumi
AU - Nagatsuka, Hitoshi
AU - Missana, Liliana Raquel
N1 - Funding Information:
This study was funded by the Japan Society for Promotion of Science (JSPS) KAKENHI Grants-in-Aid for Science Research (16K2057708, 18K0978900) and PIP-Conicet. We wish to thank Mrs. María Lilia Romano for her technical assistance.
Publisher Copyright:
© 2019, Society of Hard Tissue Regenerative Biology. All rights reserved.
PY - 2019
Y1 - 2019
N2 - In order to evaluate the biocompatibility and mineral repair capacity, mineral trioxide aggregate (MTA), portland cement normal setting (PCn) and fast setting (PCf) and calcium hydroxide-based paste (Calen) filled silicone tube were implanted into the dorsal subcutaneous connective tissues of 25 Wistar rats. Animals were euthanized at 24, 72 hours, 7, 15 and 30 days. Implants with surrounding tissues were fixed with 10% buffer formaldehyde and processed for histological routine techniques. Slides (5 µm serial cuts) were stained with H&E and Von Kossa stains for morphological, qualitative and quantitative analysis by light microscopy. Calen showed severe and moderate inflammatory response and granuloma-tous reaction with psammoma body-like formation. PCn and MTA have similar behavior, with mild inflammatory reaction from 8% and 4%, respectively. Even though, PCn and MTA demonstrated analogous biological reaction, MTA developing thick artificial mineral precipitation (p = 0,007). All sealers demonstrated a similar inflammatory response at all time periods studied (p = 0.678).
AB - In order to evaluate the biocompatibility and mineral repair capacity, mineral trioxide aggregate (MTA), portland cement normal setting (PCn) and fast setting (PCf) and calcium hydroxide-based paste (Calen) filled silicone tube were implanted into the dorsal subcutaneous connective tissues of 25 Wistar rats. Animals were euthanized at 24, 72 hours, 7, 15 and 30 days. Implants with surrounding tissues were fixed with 10% buffer formaldehyde and processed for histological routine techniques. Slides (5 µm serial cuts) were stained with H&E and Von Kossa stains for morphological, qualitative and quantitative analysis by light microscopy. Calen showed severe and moderate inflammatory response and granuloma-tous reaction with psammoma body-like formation. PCn and MTA have similar behavior, with mild inflammatory reaction from 8% and 4%, respectively. Even though, PCn and MTA demonstrated analogous biological reaction, MTA developing thick artificial mineral precipitation (p = 0,007). All sealers demonstrated a similar inflammatory response at all time periods studied (p = 0.678).
KW - Biocompatibility
KW - Calcium hydroxide
KW - Mineral trioxide aggregate
KW - Portland cement
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U2 - 10.2485/jhtb.28.1
DO - 10.2485/jhtb.28.1
M3 - Article
AN - SCOPUS:85061719604
SN - 1341-7649
VL - 28
SP - 1
EP - 6
JO - Journal of Hard Tissue Biology
JF - Journal of Hard Tissue Biology
IS - 1
ER -