TY - JOUR
T1 - Identification and purification of a Bombyx mori homologue of FTZ-F1
AU - Ueda, Hitoshi
AU - Hirose, Susumu
N1 - Funding Information:
We thank Carl Wu and Joachim Clos for technical advice for the HPLC and Kimiji Onimaru for the supply of silkworms. We also thank Sun Kansei and Tsutomu Ohta for helpful suggestion and Barbara Walker for critical reading of the munuscript. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Edcation, Scinece, and Culture of Japan (Priority area # 62124047).
PY - 1990
Y1 - 1990
N2 - Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mori contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site of FTZ-F1, a positive regulator of the fushi tarazu gene in Drosophlla melanogaster. BmFTZ-F1 and FTZ-F1 share the same methylation interference patterns, the same chromatographic behaviors and similar protease digestion profiles. Anti-FTZ-F1 cross reacts with BmFTZ-F1. These results indicate that BmFTZ-F1 is a B.mori homologue of FTZ-F1. The mobility of the factor-DNA complex formed in the silk gland extract changes depending on the developmental stages. Purification of BmFTZF1 to an almost homogeneous state reveals that the factor is a 73 kd protein.
AB - Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mori contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site of FTZ-F1, a positive regulator of the fushi tarazu gene in Drosophlla melanogaster. BmFTZ-F1 and FTZ-F1 share the same methylation interference patterns, the same chromatographic behaviors and similar protease digestion profiles. Anti-FTZ-F1 cross reacts with BmFTZ-F1. These results indicate that BmFTZ-F1 is a B.mori homologue of FTZ-F1. The mobility of the factor-DNA complex formed in the silk gland extract changes depending on the developmental stages. Purification of BmFTZF1 to an almost homogeneous state reveals that the factor is a 73 kd protein.
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U2 - 10.1093/nar/18.24.7229
DO - 10.1093/nar/18.24.7229
M3 - Article
C2 - 2124348
AN - SCOPUS:0025679208
SN - 0305-1048
VL - 18
SP - 7229
EP - 7234
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 24
ER -