TY - JOUR
T1 - G-CSF enhances stem cell proliferation in rat hippocampus after transient middle cerebral artery occlusion
AU - Sehara, Yoshihide
AU - Hayashi, Takeshi
AU - Deguchi, Kentaro
AU - Zhang, Hanzhe
AU - Tsuchiya, Atsushi
AU - Yamashita, Toru
AU - Lukic, Violeta
AU - Nagai, Makiko
AU - Kamiya, Tatsushi
AU - Abe, Koji
N1 - Funding Information:
The authors thank Dr. T. Seki (Tokyo, Japan) for his generous gift of the antibody against PSA-NCAM and Chugai Pharmaceutical Corporation Ltd. (Tokyo, Japan) for the generous gift of G-CSF. This work was partly supported by Grants-in-Aid for Scientific Research (B) 18390257, (C) 18590957 and (Hoga) 17659445 and the National Project on Protein Structural and Functional Analyses (Nakagawa A) from the Ministry of Education, Science, Culture and Sports of Japan, and by grants (Itoyama Y, Imai T and Kuzuhara S) from the Ministry of Health and Welfare of Japan.
PY - 2007/5/18
Y1 - 2007/5/18
N2 - Granulocyte colony-stimulating factor (G-CSF) enhances the survival and stimulates the proliferation of neutrophil progenitors. Recently, the neurogenerative effect of G-CSF has been intensely investigated. In this study, we explored the possibility that G-CSF enhanced the cell proliferation in the rat dentate gyrus (DG) after focal cerebral ischemia, using a rat transient middle cerebral artery occlusion (tMCAO) model. At 7 days after tMCAO, the number of 5-bromodeoxyuridine (BrdU)-positive cells in the G-CSF-treated group was significantly increased compared with that in the vehicle-treated group in the ipsilateral SGZ (16.6 ± 5.5/mm2 in the vehicle-treated group versus 33.0 ± 7.2/mm2 in the G-CSF-treated group, **p < 0.01) and in the ipsilateral GCL (14.2 ± 2.8/mm2 in the vehicle-treated group versus 21.0 ± 3.8/mm2 in the G-CSF-treated group, *p < 0.05). This result showed the possibility of a neurogenerative role of G-CSF after tMCAO in rats.
AB - Granulocyte colony-stimulating factor (G-CSF) enhances the survival and stimulates the proliferation of neutrophil progenitors. Recently, the neurogenerative effect of G-CSF has been intensely investigated. In this study, we explored the possibility that G-CSF enhanced the cell proliferation in the rat dentate gyrus (DG) after focal cerebral ischemia, using a rat transient middle cerebral artery occlusion (tMCAO) model. At 7 days after tMCAO, the number of 5-bromodeoxyuridine (BrdU)-positive cells in the G-CSF-treated group was significantly increased compared with that in the vehicle-treated group in the ipsilateral SGZ (16.6 ± 5.5/mm2 in the vehicle-treated group versus 33.0 ± 7.2/mm2 in the G-CSF-treated group, **p < 0.01) and in the ipsilateral GCL (14.2 ± 2.8/mm2 in the vehicle-treated group versus 21.0 ± 3.8/mm2 in the G-CSF-treated group, *p < 0.05). This result showed the possibility of a neurogenerative role of G-CSF after tMCAO in rats.
KW - Colony-stimulating factor
KW - Granulocyte
KW - Hippocampus
KW - Neurogenesis
KW - Transient middle cerebral artery occlusion
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U2 - 10.1016/j.neulet.2007.03.047
DO - 10.1016/j.neulet.2007.03.047
M3 - Article
C2 - 17449175
AN - SCOPUS:34247584994
SN - 0304-3940
VL - 418
SP - 248
EP - 252
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -