Estrogen favors the differentiation of ovarian tissues in the ambisexual gonads of anemonefish Amphiprion clarkii

Saori Miura, Yasuhisa Kobayashi, Ramji Kumar Bhandari, Masaru Nakamura


5 被引用数 (Scopus)


All undifferentiated gonads of anemonefish first differentiate into ovaries, and then testicular tissue appear among ovarian tissue, and finally form ambisexual gonads with both ovarian and testicular tissues. The role of estradiol-17beta (E2) in differentiation of ovarian cells is well conserved across phyla; however, its role in development of ambisexual gonads is poorly understood. Here we demonstrate that the E2 produced during the differentiation of ovarian cells does not allow testicular cells to differentiate in the prospective ambisexual gonad. We examined the immunolocalization of the steroidogenic enzyme cytochrome aromatase (P450arom), which is involved in E2 production. In the gonads, numbers of the P450arom-positive cells increased during ovarian differentiation. However, immunopositive cells with weak signal intensity were seen in the interstitial areas among oocytes and between oocytes and testicular tissue undergoing testicular differentiation. In contrast, P450arom-positive cells were not found in any testicular tissues of the ambisexual gonads. We also examined changes in E2 production in vitro in the gonads during testicular differentiation. E2 was high in the ovaries before the appearance all of testicular tissue, and decreased accompanying the differentiation of testicular tissue. These results suggest a balance of estrogen/androgen seems to be important during sex differentiation, and then a shift from estrogen to androgen production may induce testicular differentiation in the ovary. Further, exogenous E2 treatment suppressed naturally occurring differentiation of testicular cells forming exclusively ovarian tissues in the gonad in vivo, suggesting the increase of estrogen blocks the differentiation of testicular tissue and the formation of ambisexual gonad.

ジャーナルJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
出版ステータスPublished - 12月 2013

ASJC Scopus subject areas

  • 生態、進化、行動および分類学
  • 生理学
  • 動物科学および動物学
  • 分子生物学
  • 遺伝学


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