TY - JOUR
T1 - Establishment of a human pluripotent stem cell-derived MKX-td Tomato reporter system
AU - Fujisawa, Yuki
AU - Ming, Lu
AU - Yamada, Daisuke
AU - Takao, Tomoka
AU - Takarada, Takeshi
N1 - Funding Information:
The authors thank the National Research Institute for Child Health and Development (Tokyo, Japan) for providing the hESC line SEES4.
Funding Information:
This work was mainly supported by Projects for Grants-in-aid for Scientific Research from the Japan Society for the Promotion of Science (Grant No. 21H02643 to TaT) and AMED (Grant No. 18bm0704024h0001 to TaT). However, these funders played no role in the study design, data collection and analysis, publication decision, or manuscript preparation.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visualizes MKX+ cells has not been developed. Here, we established an hPSC-derived MKX-tdTomato reporter cell line and tested the induction ratio of MKX-tdTomato+ cells using our stepwise/xeno-free differentiation protocol. MKX-tdTomato+ cells were generated with high efficiency and expressed tendon-specific markers, including MKX, SCX, TNMD, and COL1A1. Our MKX-tdTomato hPSC line would be a useful tool for studying the development or regeneration of tendon tissue.
AB - Tendon regeneration is difficult because detailed knowledge about tendon progenitor cells (TPCs), which produce tenocytes to repair tendon tissue, has not been revealed. Mohawk homeobox (MKX) is a marker of TPCs or tenocytes, but a human pluripotent stem cell (hPSC)-based reporter system that visualizes MKX+ cells has not been developed. Here, we established an hPSC-derived MKX-tdTomato reporter cell line and tested the induction ratio of MKX-tdTomato+ cells using our stepwise/xeno-free differentiation protocol. MKX-tdTomato+ cells were generated with high efficiency and expressed tendon-specific markers, including MKX, SCX, TNMD, and COL1A1. Our MKX-tdTomato hPSC line would be a useful tool for studying the development or regeneration of tendon tissue.
KW - Human pluripotent stem cells
KW - Tendon
KW - Tendon regeneration
KW - Tenocytes
UR - http://www.scopus.com/inward/record.url?scp=85141720104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85141720104&partnerID=8YFLogxK
U2 - 10.1186/s13287-022-03203-5
DO - 10.1186/s13287-022-03203-5
M3 - Article
C2 - 36371298
AN - SCOPUS:85141720104
SN - 1757-6512
VL - 13
JO - Stem Cell Research and Therapy
JF - Stem Cell Research and Therapy
IS - 1
M1 - 515
ER -
