TY - JOUR
T1 - Enzymatic conversion-based method for screening cyclic dipeptide-producing microbes
AU - Arunrattiyakorn, Panarat
AU - Nitoda, Teruhiko
AU - Kanzaki, Hiroshi
N1 - Funding Information:
This work was carried out in collaboration with a scientific cooperation program between the Japan Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT) under the Core University Program on microbial resources. P.A. acknowledges the receipt of a scholarship from the Japanese Ministry of Education, Culture, Sports, Science and Technology (Monbukagakusho). We are grateful to the SC-NMR Laboratory of Okayama University and the MS Laboratory of the Faculty of Agriculture, Okayama University, for assistance in the NMR and MS experiments, respectively.
PY - 2006/4
Y1 - 2006/4
N2 - We developed a method for screening cyclic dipeptide-producing microbes by enzymatic conversion. In this method, cyclic dipeptides are detected by the combination of: (i) conversion of cyclic dipeptides to dehydro cyclic dipeptides by cyclo(Leu-Phe) oxidase and (ii) detection of the dehydro derivative by UV spectrophotometry using TLC or HPLC analysis based on the absorbance change caused by the conversion. Using this method, the actinomycete strain A8 was isolated as a cyclic dipeptide-producing strain. The cyclic dipeptides were purified from the microbial extract by enzymatic detection-guided fractionation, and their structures were determined to be cyclo(l-Phe-l-Pro) and cyclo(l-Pro-l-Tyr) by spectroscopic methods.
AB - We developed a method for screening cyclic dipeptide-producing microbes by enzymatic conversion. In this method, cyclic dipeptides are detected by the combination of: (i) conversion of cyclic dipeptides to dehydro cyclic dipeptides by cyclo(Leu-Phe) oxidase and (ii) detection of the dehydro derivative by UV spectrophotometry using TLC or HPLC analysis based on the absorbance change caused by the conversion. Using this method, the actinomycete strain A8 was isolated as a cyclic dipeptide-producing strain. The cyclic dipeptides were purified from the microbial extract by enzymatic detection-guided fractionation, and their structures were determined to be cyclo(l-Phe-l-Pro) and cyclo(l-Pro-l-Tyr) by spectroscopic methods.
KW - Cyclo(Leu-Phe) oxidase
KW - Dehydro cyclic dipeptide
KW - Diketopiperazine
KW - Enzymatic conversion-based detection
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U2 - 10.1016/j.peptides.2005.08.017
DO - 10.1016/j.peptides.2005.08.017
M3 - Article
C2 - 16226343
AN - SCOPUS:33645381483
SN - 0196-9781
VL - 27
SP - 633
EP - 639
JO - Peptides
JF - Peptides
IS - 4
ER -