Elevation of glutathione in RAW 264.7 cells by low-dose γ-ray irradiation and its responsibility for the appearance of radioresistance

Shuji Kojima, Shirane Matsumori, Hideki Ono, Kiyonori Yamaoka

研究成果査読

12 被引用数 (Scopus)

抄録

We examined the relationship between the induction of glutathione (GSH) level in macrophage-like RAW264.7 cells by a low (adapting) dose γ-rays and the cell damage caused by a lethal dose of γ-rays at various intervals after the adapting dose. The reduced glutathione (GSH) level increased soon after exposure of the cells to 25 cGy of γ-rays, peaked between 3 hr and 6 hr, and returned almost to the zero time (0 hr) level by 24 hr post-irradiation. Cell damage was assessed by measuring the 3H-thymidine (3H-TdR) incorporation into cellular DNA. γ-Ray irradiation produced a dose-dependent cell damage in RAW cells, causing about 40% and 60% inhibition of 3H-TdR incorporation into DNA at 1.0 Gy and 2.0 Gy, respectively, as compared with non-irradiated cells. Treatment with the adapting dose of 25 cGy at 1 hr or 24 hr before the lethal irradiation was ineffective. However, pre-irradiation with 25 cGy at 3 hr or 6 hr prior to lethal irradiation inhibited the decrease of 3H-TdR incorporation into DNA, indicating a protective effect. GSH exogenously added to the medium also inhibited the cell damage induced by lethal doses of γ-Rays in a dose-dependent manner. These results indicate that the induction of endogenous GSH in living cells immediately following low-dose γ-Ray irradiation is at least partially responsible for the appearance of radioresistance to a subsequent lethal dose of radiation, and may make it possible to use higher doses of radiation in radiotherapy for tumor patients.

本文言語English
ページ(範囲)5271-5275
ページ数5
ジャーナルAnticancer research
19
6 B
出版ステータスPublished - 11月 1 1999

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

フィンガープリント

「Elevation of glutathione in RAW 264.7 cells by low-dose γ-ray irradiation and its responsibility for the appearance of radioresistance」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル