TY - JOUR
T1 - Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken
AU - Yokoyama, Shigetoshi
AU - Hashimoto, Megumi
AU - Shimizu, Hirohito
AU - Ueno-Kudoh, Hiroe
AU - Uchibe, Kenta
AU - Kimura, Ichiro
AU - Asahara, Hiroshi
N1 - Funding Information:
We thank Dr. Hayashizaki for providing the FANTOM cDNA library. We also thank Akane Nagai, Tomohiro Hikata, Hironobu Naito, Takamasa Suzuki, and Noritsugu Fukuda for technical assistance and critical discussion, and all members of the Asahara laboratory for helpful discussion. This study was supported by Grants from NIH (AR50631), JST SORST, Grant ID05-24 from the National Institute of Biomedical Innovation, Genome Network Project (MEXT) and DECODE Grants-in-Aid for Scientific Research (MEXT) (H.A.)
PY - 2008/2
Y1 - 2008/2
N2 - The Caenorhabditis elegans heterochronic gene lin-28 regulates developmental timing in the nematode trunk. We report the dynamic expression patterns of Lin-28 homologues in mouse and chick embryos. Whole mount in situ hybridization revealed specific and intriguing expression patterns of Lin-28 in the developing mouse and chick limb bud. Mouse Lin-28 expression was detected in both the forelimb and hindlimb at E9.5, but disappeared from the forelimb at E10.5, and finally from the forelimb and hindlimb at E11.5. Chicken Lin-28, which was first detected in the limb primordium at stage 15/16, was also downregulated as the stage proceeded. The amino acid sequences of mouse and chicken Lin-28 genes are highly conserved and the similar expression patterns of Lin-28 during limb development in mouse and chicken suggest that this heterochronic gene is also conserved during vertebrate limb development.
AB - The Caenorhabditis elegans heterochronic gene lin-28 regulates developmental timing in the nematode trunk. We report the dynamic expression patterns of Lin-28 homologues in mouse and chick embryos. Whole mount in situ hybridization revealed specific and intriguing expression patterns of Lin-28 in the developing mouse and chick limb bud. Mouse Lin-28 expression was detected in both the forelimb and hindlimb at E9.5, but disappeared from the forelimb at E10.5, and finally from the forelimb and hindlimb at E11.5. Chicken Lin-28, which was first detected in the limb primordium at stage 15/16, was also downregulated as the stage proceeded. The amino acid sequences of mouse and chicken Lin-28 genes are highly conserved and the similar expression patterns of Lin-28 during limb development in mouse and chicken suggest that this heterochronic gene is also conserved during vertebrate limb development.
KW - Chick
KW - Developmental timing
KW - Dynamic expression
KW - Embryo
KW - Heterochronic gene
KW - Limb bud
KW - Lin-28
KW - MicroRNAs
KW - Mouse
KW - Whole mount in situ hybridization
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U2 - 10.1016/j.gep.2007.11.001
DO - 10.1016/j.gep.2007.11.001
M3 - Article
C2 - 18077221
AN - SCOPUS:38049085393
SN - 1567-133X
VL - 8
SP - 155
EP - 160
JO - Gene Expression Patterns
JF - Gene Expression Patterns
IS - 3
ER -