@article{c5b67e13d3cd4c3f81983ae090173e62,
title = "Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: In vitro assay system for plant cPNGase activity",
abstract = "Cytosolic peptide:N-glycanase (cPNGase), which occurs ubiquitously in eukaryotic cells, is involved in the de-N-glycosylation of misfolded glycoproteins in the protein quality control system. In this study, we aimed to provide direct evidence of plant cPNGase activity against a denatured glycoprotein using a crude extract prepared from a mutant line of Arabidopsis thaliana lacking 2 acidic PNGase genes. ",
keywords = "Arabidopsis thaliana, PNGase assay, acidic PNGase, cytosolic PNGase, free N-glycan",
author = "Sahoko Shirai and Ryota Uemura and Megumi Maeda and Hiroyuki Kajiura and Ryo Misaki and Kazuhito Fujiyama and Yoshinobu Kimura",
note = "Publisher Copyright: {\textcopyright} 2021 The Author(s). Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.",
year = "2021",
month = jun,
day = "1",
doi = "10.1093/bbb/zbab047",
language = "English",
volume = "85",
pages = "1460--1463",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "6",
}