Determination of amino acid sequence and site of mRNA expression of four vitellins in the giant freshwater prawn, Macrobrachium rosenbergii

Wei Jun Yang, Tsuyoshi Ohira, Naoaki Tsutsui, Thanumalayaperumal Subramoniam, Do Thi Thanh Huong, Katsumi Aida, Marcy N. Wilder

研究成果査読

81 被引用数 (Scopus)

抄録

Four major yolk proteins, designated as vitellins (Vns) Macr-VnA, B, C, and D, were extracted from mature ovaries of Macrobrachium rosenbergii. These were purified to homogeneity by reversed-phase high performance liquid chromatography (HPLC) employing a unique separation system based on the hydrophobic properties of the Vn molecule. Using standard techniques of protein sequencing, more than 33 N-terminal and 57 internal amino acid residues were determined for each of the four Vns. The cDNA fragments encoding the four Vns were amplified by PCR using degenerate oligonucleotide primers derived from the N-terminal and internal amino acid sequences. These cDNA fragments were cloned, sequenced, and used as probes to examine the transcription of mRNAs encoding the four Vns. Significant accumulations of these mRNAs were observed in female hepatopancreas only, while mRNA expression was not detected in male hepatopancreas or any other female tissue including ovary, subepidermal adipose tissue, gill, and muscle. This is the first occasion in Crustacea in which multiple Vns were demonstrated to be synthesized simultaneously in a single tissue. (C) 2000 Wiley-Liss, Inc.

本文言語English
ページ(範囲)413-422
ページ数10
ジャーナルJournal of Experimental Zoology
287
6
DOI
出版ステータスPublished - 11月 1 2000

ASJC Scopus subject areas

  • 動物科学および動物学

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