TY - JOUR
T1 - Detection of novel orthoreovirus genomes in shrew (Crocidura hirta) and fruit bat (rousettus aegyptiacus)
AU - Harima, Hayato
AU - Sasaki, Michihito
AU - Kajihara, Masahiro
AU - Mori-Kajihara, Akina
AU - Hang’ombe, Bernard M.
AU - Changula, Katendi
AU - Orba, Yasuko
AU - Ogawa, Hirohito
AU - Simuunza, Martin
AU - Yoshida, Reiko
AU - Mweene, Aaron
AU - Takada, Ayato
AU - Sawa, Hirofumi
N1 - Funding Information:
We would like to thank Dr. Masayuki Saijo and Dr. Satoshi Taniguchi from the National Institute of Infectious Diseases, Japan for for providing orthoreovirus RNA. This study was supported by grants for Grant-in-Aid Scientific Research (B) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)/Japan Society for the Promotion of Science (JSPS) KAKENHI (JP16H05805); and grants for Scientific Research on Innovative Areas and International Group from the MEXT/JSPS KAKENHI (JP16H06431, JP16H06429, JP16K21723), and the Japan Initiative for Global Research Network of Infectious Diseases (J-GRID) from Japan Agency for Medical Research and Development (AMED) (JP19fm0108008); and grants for the AMED and Japan International Cooperation Agency (JICA) within the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS) (JP19jm0110019).
Funding Information:
ACKNOWLEDGMENTS. We would like to thank Dr. Masayuki Saijo and Dr. Satoshi Taniguchi from the National Institute of Infectious Diseases, Japan for for providing orthoreovirus RNA. This study was supported by grants for Grant-in-Aid Scientific Research (B) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)/Japan Society for the Promotion of Science (JSPS) KAKENHI (JP16H05805); and grants for Scientific Research on Innovative Areas and International Group from the MEXT/JSPS KAKENHI (JP16H06431, JP16H06429, JP16K21723), and the Japan Initiative for Global Research Network of Infectious Diseases (J-GRID) from Japan Agency for Medical Research and Development (AMED) (JP19fm0108008); and grants for the AMED and Japan International Cooperation Agency (JICA) within the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS) (JP19jm0110019).
Publisher Copyright:
© 2020 The Japanese Society of Veterinary Science.
PY - 2020
Y1 - 2020
N2 - Orthoreoviruses have been indentified in several mammals, however, there is no information about orthoreoviruses in shrews. In this study, we screened wild animals in Zambia, including shrews, rodents, and bats for the detection of orthoreoviruses. Two orthoreovirus RNA genomes were detected from a shrew intestinal-contents (1/24) and a bat colon (1/96) sample by reverse-transcription (RT)-PCR targeting the RNA-dependent RNA polymerase gene of orthoreoviruses. Phylogenetic analyses revealed that each of the identified orthoreoviruses formed a distinct branch among members of the Orthoreovirus genus. This is the first report that shrews are susceptible to orthoreovirus infection. Our results suggest the existence of undiscovered orthoreoviruses in shrews and provide important information about the genetic diversity of orthoreoviruses.
AB - Orthoreoviruses have been indentified in several mammals, however, there is no information about orthoreoviruses in shrews. In this study, we screened wild animals in Zambia, including shrews, rodents, and bats for the detection of orthoreoviruses. Two orthoreovirus RNA genomes were detected from a shrew intestinal-contents (1/24) and a bat colon (1/96) sample by reverse-transcription (RT)-PCR targeting the RNA-dependent RNA polymerase gene of orthoreoviruses. Phylogenetic analyses revealed that each of the identified orthoreoviruses formed a distinct branch among members of the Orthoreovirus genus. This is the first report that shrews are susceptible to orthoreovirus infection. Our results suggest the existence of undiscovered orthoreoviruses in shrews and provide important information about the genetic diversity of orthoreoviruses.
KW - Crocidura hirta
KW - Orthoreovirus
KW - Rousettus aegyptiacus
KW - Zambia
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U2 - 10.1292/jvms.19-0424
DO - 10.1292/jvms.19-0424
M3 - Article
C2 - 31866632
SN - 0916-7250
VL - 82
SP - 162
EP - 167
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
IS - 2
ER -