TY - JOUR
T1 - Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation
AU - Yasui, Norihisa
AU - Mihara, Emiko
AU - Nampo, Maiko
AU - Tamura-Kawakami, Keiko
AU - Unno, Hideaki
AU - Matsumoto, Kyoichi
AU - Takagi, Junichi
N1 - Funding Information:
We thank Shigemichi Nishikawa and Takeshi Kubo at Wakenyaku Co., Ltd for the large scale cell culture and Mayumi Nakano for the preparation of the manuscript. This work was supported in part by the Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) .
PY - 2010/1/31
Y1 - 2010/1/31
N2 - LRP6 is a cell surface molecule that plays a critical role in the Wnt signaling pathway, and is implicated in numerous human diseases. Studies of cellular signaling mediated by LRP6 have relied on overexpression experiments, due to the lack of good monoclonal antibodies (mAbs) reactive with native LRP6 ectodomain. By using native recombinant LRP6 ectodomain fragment produced in mammalian expression system, we succeeded in developing a panel of anti-human LRP6 mAbs. Selected mAbs were capable of staining endogenous LRP6 on cell surface by using flow cytometry and immunofluorescence microscopy, and enriching detergent-solubilized LRP6 from cell lysate by immunoprecipitation.
AB - LRP6 is a cell surface molecule that plays a critical role in the Wnt signaling pathway, and is implicated in numerous human diseases. Studies of cellular signaling mediated by LRP6 have relied on overexpression experiments, due to the lack of good monoclonal antibodies (mAbs) reactive with native LRP6 ectodomain. By using native recombinant LRP6 ectodomain fragment produced in mammalian expression system, we succeeded in developing a panel of anti-human LRP6 mAbs. Selected mAbs were capable of staining endogenous LRP6 on cell surface by using flow cytometry and immunofluorescence microscopy, and enriching detergent-solubilized LRP6 from cell lysate by immunoprecipitation.
KW - LRP6
KW - Monoclonal antibody
KW - Wnt
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U2 - 10.1016/j.jim.2009.11.016
DO - 10.1016/j.jim.2009.11.016
M3 - Article
C2 - 19945460
AN - SCOPUS:71749096157
SN - 0022-1759
VL - 352
SP - 153
EP - 160
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -
