TY - JOUR
T1 - Defective adipose tissue development associated with hepatomegaly in cathepsin E-deficient mice fed a high-fat diet
AU - Kadowaki, Tomoko
AU - Kido, Mizuho A.
AU - Hatakeyama, Junko
AU - Okamoto, Kuniaki
AU - Tsukuba, Takayuki
AU - Yamamoto, Kenji
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (T.T.). We would like to thank Prof. Keiichi I. Nakayama (Kyushu University, Department of Molecular and Cellular Biology, Medical Institute of Bioregulation) for generating the CatE −/− mice.
PY - 2014/3/28
Y1 - 2014/3/28
N2 - Cathepsin E is an intracellular aspartic proteinase, which is predominantly distributed in immune-related and epithelial cells. However, the role of the enzyme in adipose tissues remains unknown. In this study, we investigated the characteristics of cathepsin E-deficient (CatE-/-) mice fed a high-fat diet (HFD), as a mouse model of obesity. HFD-fed CatE-/- mice displayed reduced body weight gain and defective development of white adipose tissue (WAT) and brown adipose tissue (BAT), compared with HFD-fed wild-type mice. Moreover, fat-induced CatE-/- mice showed abnormal lipid accumulation in non-adipose tissues characterized by hepatomegaly, which is probably due to defective adipose tissue development. Detailed pathological and biochemical analyses showed that hepatomegaly was accompanied by hepatic steatosis and hypercholesterolemia in HFD-induced CatE-/- mice. In fat-induced CatE-/- mice, the number of macrophages infiltrating into WAT was significantly lower than in fat-induced wild-type mice. Thus, the impaired adipose tissue development in HFD-induced CatE-/- mice was probably due to reduced infiltration of macrophages and may lead to hepatomegaly accompanied by hepatic steatosis and hypercholesterolemia.
AB - Cathepsin E is an intracellular aspartic proteinase, which is predominantly distributed in immune-related and epithelial cells. However, the role of the enzyme in adipose tissues remains unknown. In this study, we investigated the characteristics of cathepsin E-deficient (CatE-/-) mice fed a high-fat diet (HFD), as a mouse model of obesity. HFD-fed CatE-/- mice displayed reduced body weight gain and defective development of white adipose tissue (WAT) and brown adipose tissue (BAT), compared with HFD-fed wild-type mice. Moreover, fat-induced CatE-/- mice showed abnormal lipid accumulation in non-adipose tissues characterized by hepatomegaly, which is probably due to defective adipose tissue development. Detailed pathological and biochemical analyses showed that hepatomegaly was accompanied by hepatic steatosis and hypercholesterolemia in HFD-induced CatE-/- mice. In fat-induced CatE-/- mice, the number of macrophages infiltrating into WAT was significantly lower than in fat-induced wild-type mice. Thus, the impaired adipose tissue development in HFD-induced CatE-/- mice was probably due to reduced infiltration of macrophages and may lead to hepatomegaly accompanied by hepatic steatosis and hypercholesterolemia.
KW - Adipose tissue
KW - Cathepsin E
KW - High-fat diet
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U2 - 10.1016/j.bbrc.2014.02.089
DO - 10.1016/j.bbrc.2014.02.089
M3 - Article
C2 - 24583126
AN - SCOPUS:84897990214
SN - 0006-291X
VL - 446
SP - 212
EP - 217
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -
