Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum

Yuuka Kimura, Tomoaki Kawakami, Long Jiang Yu, Miku Yoshimura, Masayuki Kobayashi, Zheng Yu Wang-Otomo

研究成果査読

13 被引用数 (Scopus)

抄録

Abstract Quinone distributions in the thermophilic purple sulfur bacterium Thermochromatium tepidum have been investigated at different levels of the photosynthetic apparatus. Here we show that, on average, the intracytoplasmic membrane contains 18 ubiquinones (UQ) and 4 menaquinones (MQ) per reaction center (RC). About one-third of the quinones are retained in the light-harvesting-reaction center core complex (LH1-RC) with a similar ratio of UQ to MQ. The numbers of quinones essentially remains unchanged during crystallization of the LH1-RC. There are 1-2 UQ and 1 MQ associated with the RC-only complex in the purified solution sample. Our results suggest that a large proportion of the quinones are confined to the core complex and at least five UQs remain invisible in the current LH1-RC crystal structure.

本文言語English
論文番号37199
ページ(範囲)1761-1765
ページ数5
ジャーナルFEBS Letters
589
15
DOI
出版ステータスPublished - 7月 3 2015

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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