TY - JOUR
T1 - Calcium-binding protein-immunoreactive innervation of the rat vibrissa
AU - Ichikawa, H.
AU - Sugimoto, T.
N1 - Funding Information:
We greatly appreciate Dr James I. Morgan at the Department of Developmental Neurobiology, St. Jude Children’s Research Hospital and Dr. Hiroyoshi Hidaka at the Department of Pharmacology, Nagoya University School of Medicine for their kind gift of the antisera to PEP19 and NC, respectively. This work was supported by a grant from the Japanese Ministry of Education, Culture, Sports, Science and Technology to H.I. (No. 14571733).
PY - 2003/4/25
Y1 - 2003/4/25
N2 - Immunohistochemistry detected calcium-binding proteins (CaBPs) in corpuscular and Merkel nerve endings of the rat vibrissa. CaBP-immunoreactive (ir) corpuscular endings were divided into two types: ramified and unramified endings. Ramified endings were subdivided into reticular and Ruffini endings. Unramified endings were identical to longitudinal lanceolate endings which have been described previously. Reticular and unramified endings as well as Merkel endings co-expressed neurocalcin (NC)- and parvalbumin (PV)-immunoreactivity (ir). However, such endings were devoid of peptide 19 (PEP19)-ir. PV-ir Ruffini endings were immunoreactive for PEP19 but not NC. The retrograde tracing method revealed that 34, 21 and 18% of trigeminal neurons which project to the infraorbital nerve exhibited NC-, PEP19- and PV-ir, respectively. In addition, 73 and 36% of the PV-ir neurons showed NC- and PEP19-ir, respectively. The content and co-expression of CaBPs in vibrissal low-threshold mechanoreceptors may depend on their terminal morphology.
AB - Immunohistochemistry detected calcium-binding proteins (CaBPs) in corpuscular and Merkel nerve endings of the rat vibrissa. CaBP-immunoreactive (ir) corpuscular endings were divided into two types: ramified and unramified endings. Ramified endings were subdivided into reticular and Ruffini endings. Unramified endings were identical to longitudinal lanceolate endings which have been described previously. Reticular and unramified endings as well as Merkel endings co-expressed neurocalcin (NC)- and parvalbumin (PV)-immunoreactivity (ir). However, such endings were devoid of peptide 19 (PEP19)-ir. PV-ir Ruffini endings were immunoreactive for PEP19 but not NC. The retrograde tracing method revealed that 34, 21 and 18% of trigeminal neurons which project to the infraorbital nerve exhibited NC-, PEP19- and PV-ir, respectively. In addition, 73 and 36% of the PV-ir neurons showed NC- and PEP19-ir, respectively. The content and co-expression of CaBPs in vibrissal low-threshold mechanoreceptors may depend on their terminal morphology.
KW - Immunohistochemistry
KW - Neurocalcin
KW - Parvalbumin
KW - Peptide 19
KW - Trigeminal ganglion
KW - Vibrissa
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U2 - 10.1016/S0006-8993(03)02292-3
DO - 10.1016/S0006-8993(03)02292-3
M3 - Article
C2 - 12706265
AN - SCOPUS:0037466344
SN - 0006-8993
VL - 970
SP - 226
EP - 231
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -