@article{c0c9c84ed9a845d081e3ff5dc109bf26,
title = "Architecture of the chloroplast PSI–NDH supercomplex in Hordeum vulgare",
abstract = "The chloroplast NADH dehydrogenase-like (NDH) complex is composed of at least 29 subunits and has an important role in mediating photosystem I (PSI) cyclic electron transport (CET)1–3. The NDH complex associates with PSI to form the PSI–NDH supercomplex and fulfil its function. Here, we report cryo-electron microscopy structures of a PSI–NDH supercomplex from barley (Hordeum vulgare). The structures reveal that PSI–NDH is composed of two copies of the PSI–light-harvesting complex I (LHCI) subcomplex and one NDH complex. Two monomeric LHCI proteins, Lhca5 and Lhca6, mediate the binding of two PSI complexes to NDH. Ten plant chloroplast-specific NDH subunits are presented and their exact positions as well as their interactions with other subunits in NDH are elucidated. In all, this study provides a structural basis for further investigations on the functions and regulation of PSI–NDH-dependent CET.",
author = "Liangliang Shen and Kailu Tang and Wenda Wang and Chen Wang and Hangjun Wu and Zhiyuan Mao and Shaoya An and Shenghai Chang and Tingyun Kuang and Shen, {Jian Ren} and Guangye Han and Xing Zhang",
note = "Funding Information: Acknowledgements We thank C. Ma from the Protein Facility (School of Medicine, Zhejiang University) for providing the platform for sample purification, X. Meng in the Center of Biomedical Analysis (Tsinghua University) for protein mass spectrometry analysis and W. Tang from the Institute of Botany (CAS) for instrumental support in sample preparation. The project was funded by the National Key R&D Program of China (2020YFA0907600, 2018YFA0507700, 2017YFA0503700, 2017YFA0504803, 2019YFA0906300, 2021YFA1300403), the Strategic Priority Research Program of CAS (XDA26050402, XDB17000000), the Key Research Program of Frontier Sciences of CAS (QYZDY-SSW-SMC003), the Youth Innovation Promotion Association of CAS (2020081), the CAS Interdisciplinary Innovation Team (JCTD-2020-06), the CAS Project for Young Scientists in Basic Research (YSBR-004), the Fundamental Research Funds for the Central Universities (2018XZZX001–13) and JSPS KAKENHI (JP17H06434). Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2022",
month = jan,
day = "27",
doi = "10.1038/s41586-021-04277-6",
language = "English",
volume = "601",
pages = "649--654",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7894",
}