TY - JOUR
T1 - Antisense oligodeoxynucleotides
T2 - useful tool for search and assessment of new targets for anti-malarial drugs.
AU - Ikemoto, N.
AU - Kim, H. S.
AU - Kanazaki, M.
AU - Ueno, Y.
AU - Shuto, S.
AU - Matsuda, A.
AU - Wataya, Y.
PY - 1999
Y1 - 1999
N2 - We investigated about targeting for new antimalarial drugs using antisense (AS) oligodeoxynucleotides (ODNs). Synthetic nuclease-resistant ODNs (phosphorothioate (PS) ODNs and ODNs containing 4'alpha-C-(2-aminoethyl)thymidines (4'-amino ODNs)) which target mitochondrial succinate dehydrogenase (SDH) iron-sulfur subunit (IP), had antimalarial activity (EC50; about 1.0 microM). Furthermore we showed that intra-parasitic SDH IP mRNA levels, which were detected using quantitative RT-PCR assay, were decreased 13% of control after the 24 h expose to SDH IP AS. From the results, we conclude that SDH has potential as the target for novel antimalarials, and AS ODNs is effective for search and assessment of targets for new antimalarial drugs.
AB - We investigated about targeting for new antimalarial drugs using antisense (AS) oligodeoxynucleotides (ODNs). Synthetic nuclease-resistant ODNs (phosphorothioate (PS) ODNs and ODNs containing 4'alpha-C-(2-aminoethyl)thymidines (4'-amino ODNs)) which target mitochondrial succinate dehydrogenase (SDH) iron-sulfur subunit (IP), had antimalarial activity (EC50; about 1.0 microM). Furthermore we showed that intra-parasitic SDH IP mRNA levels, which were detected using quantitative RT-PCR assay, were decreased 13% of control after the 24 h expose to SDH IP AS. From the results, we conclude that SDH has potential as the target for novel antimalarials, and AS ODNs is effective for search and assessment of targets for new antimalarial drugs.
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U2 - 10.1093/nass/42.1.89
DO - 10.1093/nass/42.1.89
M3 - Article
C2 - 10780393
AN - SCOPUS:0033287409
SN - 0261-3166
SP - 89
EP - 90
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
IS - 42
ER -