@article{b6c804cf600b47b181012813c11ddce1,
title = "A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells",
abstract = "Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1+ limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022).",
keywords = "Cell culture, Cell differentiation, Developmental biology, Organoids, Stem cells, Tissue engineering",
author = "Tomoka Takao and Daisuke Yamada and Takeshi Takarada",
note = "Funding Information: The authors thank the Center for iPSC Research and Application and Kyoto University for providing the hPSC lines. Preparation of slides and staining was supported by the Central Research Laboratory, Okayama University Medical School. This work was mainly supported by Projects for Grants-in-aid for Scientific Research from the Japan Society for the Promotion of Science (grant no. 21H02643 to T. Takarada) and AMED (grant numbers 18bm0704024h0001, 20bm0404064h0001 to T. Takarada). T. Takao and D.Y. performed the experiments. T. Takao wrote the original draft. T. Takao, D.Y. and T. Takarada reviewed and edited the manuscript. T. Takarada supervised the study and acquired funding. T. Takao, D.Y. and T. Takarada have a patent pending related to this work (PCT/JP2020/03551). Funding Information: The authors thank the Center for iPSC Research and Application and Kyoto University for providing the hPSC lines. Preparation of slides and staining was supported by the Central Research Laboratory , Okayama University Medical School . This work was mainly supported by Projects for Grants-in-aid for Scientific Research from the Japan Society for the Promotion of Science (grant no. 21H02643 to T. Takarada) and AMED (grant numbers 18bm0704024h0001 , 20bm0404064h0001 to T. Takarada). Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = dec,
day = "16",
doi = "10.1016/j.xpro.2022.101786",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",
}