A comparative study of the reduction of EPR signal IIslow by iodide and the iodo-labeling of the D2-protein in photosystem II

Yuichiro Takahashi, Stenbjörn Styring

研究成果査読

10 被引用数 (Scopus)

抄録

The reduction kinetics of EPR signal IIslow in the presence of iodide were measured with EPR spectroscopy and the amount of 125I incorporated into the D2-protein in the reaction center of photosystem II was quantitated in a PS II core complex preparation. The halftime of signal IIslow reduction in the dark was estimated at 31, 25 and 20 min in the presence of 0, 20 and 50 mM KI, respectively, reflecting the reduction of signal IIslow by iodide. The amount of 125I that was incorporated into the D2-protein in the dark was correlated to the reduction of signal IIslow by iodide. This correlation indicates that the site of iodide oxidation in the dark and the component giving rise to EPR signal IIslow are located on the D2-protein in the PS II reaction center.

本文言語English
ページ(範囲)371-375
ページ数5
ジャーナルFEBS Letters
223
2
DOI
出版ステータスPublished - 11月 2 1987

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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