Zinc hydroxide stimulates superoxide production by rat alveolar macrophages

Keiki Ogino, Yukie Izumi, Hironobu Ishiyama, Tomoko Murata, Haruo Kobayashi, Tatsuya Houbara

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effect of zinc hydroxide on superoxide (O2-) production by rat alveolar macrophages was determined by chemiluminescence and by cytochrome c reduction. Zinc ions had no effect on the chemiluminescence of unstimulated alveolar macrophages. By contrast, zinc hydroxide (ZnOH2), a neutralized form of zinc ions, increased the chemiluminescence level and O2- release. Increased O2- release was inhibited by pertussis toxin, isoquinoline sulfonamide and pretreatment with EGTA. These findings indicate that zinc hydroxide formation from zinc compounds can stimulate the O2- production by alveolar macrophages by receptor-mediated and Ca2+-dependent process.

Original languageEnglish
Pages (from-to)1115-1121
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume185
Issue number3
DOIs
Publication statusPublished - Jun 30 1992
Externally publishedYes

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Chemiluminescence
Alveolar Macrophages
Luminescence
Superoxides
Rats
Zinc
Zinc Compounds
Ions
Egtazic Acid
Sulfonamides
Pertussis Toxin
Cytochromes c
zinc hydroxide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Zinc hydroxide stimulates superoxide production by rat alveolar macrophages. / Ogino, Keiki; Izumi, Yukie; Ishiyama, Hironobu; Murata, Tomoko; Kobayashi, Haruo; Houbara, Tatsuya.

In: Biochemical and Biophysical Research Communications, Vol. 185, No. 3, 30.06.1992, p. 1115-1121.

Research output: Contribution to journalArticle

Ogino, Keiki ; Izumi, Yukie ; Ishiyama, Hironobu ; Murata, Tomoko ; Kobayashi, Haruo ; Houbara, Tatsuya. / Zinc hydroxide stimulates superoxide production by rat alveolar macrophages. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 185, No. 3. pp. 1115-1121.
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