Zinc hydroxide induced respiratory burst in rat neutrophils

Keiki Ogino, Yukie Izumi, Hiroyuki Segawa, Yuko Takeyama, Hironobu Ishiyama, Tatsuya Houbara, Taizo Uda, Shuji Yamashita

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16 Citations (Scopus)

Abstract

The effects of zinc hydroxide on the respiratory burst and phagocytosis by rat neutrophils were examined. Zinc hydroxide induced an increase in oxygen consumption and O2- production. Electronmicroscopy showed that neutrophils engulfed zinc hydroxide particles by phagocytosis. Pertussis toxin (0.25, 0.5, 1.0 μ/ml) and EGTA (1, 2, 5 mM) inhibited zinc hydroxide-induced O2- production in a dose-dependent manner. The inhibitors of protein kinase C, 1-(5-isoquinolinesulfonyl)-2-methyl-piperazine and N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide inhibited zinc hydroxide-induced O2- production with IC50 values ranging between 10 μM and 25 μM. The inhibitory study using an inhibitor of myosin light chain kinase, 1-(5-iodonapthalene-1-sulfonyl)-1H- hexahydro-1,4-diazepine, showed IC50 values ranging from 5 μM to 10 μM. These findings indicate that zinc hydroxide induces respiratory burst and phagocytosis by rat neutrophils.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalEuropean Journal of Pharmacology: Environmental Toxicology and
Volume270
Issue number1
DOIs
Publication statusPublished - Jan 3 1994

Keywords

  • Neutrophil, rat
  • Phagocytosis
  • Respiratory burst
  • Zinc hydroxide

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Pollution

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    Ogino, K., Izumi, Y., Segawa, H., Takeyama, Y., Ishiyama, H., Houbara, T., Uda, T., & Yamashita, S. (1994). Zinc hydroxide induced respiratory burst in rat neutrophils. European Journal of Pharmacology: Environmental Toxicology and, 270(1), 73-78. https://doi.org/10.1016/0926-6917(94)90082-5