Zinc hydroxide induced respiratory burst in rat neutrophils

Keiki Ogino, Yukie Izumi, Hiroyuki Segawa, Yuko Takeyama, Hironobu Ishiyama, Tatsuya Houbara, Taizo Uda, Shuji Yamashita

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effects of zinc hydroxide on the respiratory burst and phagocytosis by rat neutrophils were examined. Zinc hydroxide induced an increase in oxygen consumption and O2- production. Electronmicroscopy showed that neutrophils engulfed zinc hydroxide particles by phagocytosis. Pertussis toxin (0.25, 0.5, 1.0 μ/ml) and EGTA (1, 2, 5 mM) inhibited zinc hydroxide-induced O2- production in a dose-dependent manner. The inhibitors of protein kinase C, 1-(5-isoquinolinesulfonyl)-2-methyl-piperazine and N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide inhibited zinc hydroxide-induced O2- production with IC50 values ranging between 10 μM and 25 μM. The inhibitory study using an inhibitor of myosin light chain kinase, 1-(5-iodonapthalene-1-sulfonyl)-1H- hexahydro-1,4-diazepine, showed IC50 values ranging from 5 μM to 10 μM. These findings indicate that zinc hydroxide induces respiratory burst and phagocytosis by rat neutrophils.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalEuropean Journal of Pharmacology: Environmental Toxicology and
Volume270
Issue number1
DOIs
Publication statusPublished - Jan 3 1994
Externally publishedYes

Fingerprint

Respiratory Burst
Rats
Neutrophils
Zinc
Phagocytosis
Inhibitory Concentration 50
Myosin-Light-Chain Kinase
Egtazic Acid
Pertussis Toxin
Oxygen Consumption
Protein Kinase C
zinc hydroxide
Oxygen
Proteins

Keywords

  • Neutrophil, rat
  • Phagocytosis
  • Respiratory burst
  • Zinc hydroxide

ASJC Scopus subject areas

  • Pollution
  • Pharmacology
  • Toxicology

Cite this

Ogino, K., Izumi, Y., Segawa, H., Takeyama, Y., Ishiyama, H., Houbara, T., ... Yamashita, S. (1994). Zinc hydroxide induced respiratory burst in rat neutrophils. European Journal of Pharmacology: Environmental Toxicology and, 270(1), 73-78. https://doi.org/10.1016/0926-6917(94)90082-5

Zinc hydroxide induced respiratory burst in rat neutrophils. / Ogino, Keiki; Izumi, Yukie; Segawa, Hiroyuki; Takeyama, Yuko; Ishiyama, Hironobu; Houbara, Tatsuya; Uda, Taizo; Yamashita, Shuji.

In: European Journal of Pharmacology: Environmental Toxicology and, Vol. 270, No. 1, 03.01.1994, p. 73-78.

Research output: Contribution to journalArticle

Ogino, K, Izumi, Y, Segawa, H, Takeyama, Y, Ishiyama, H, Houbara, T, Uda, T & Yamashita, S 1994, 'Zinc hydroxide induced respiratory burst in rat neutrophils', European Journal of Pharmacology: Environmental Toxicology and, vol. 270, no. 1, pp. 73-78. https://doi.org/10.1016/0926-6917(94)90082-5
Ogino, Keiki ; Izumi, Yukie ; Segawa, Hiroyuki ; Takeyama, Yuko ; Ishiyama, Hironobu ; Houbara, Tatsuya ; Uda, Taizo ; Yamashita, Shuji. / Zinc hydroxide induced respiratory burst in rat neutrophils. In: European Journal of Pharmacology: Environmental Toxicology and. 1994 ; Vol. 270, No. 1. pp. 73-78.
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