Zinc-finger-based artificial transcription factors and their applications

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Artificial transcription factors (ATFs) are potentially a powerful molecular tool to modulate endogenous target gene expression in living cells and organisms. To date, many DNA-binding molecules have been developed as the DNA-binding domains for ATFs. Among them, ATFs comprising Cys2His2-type zinc-finger proteins (ZFPs) as the DNA-binding domain have been extensively explored. The zinc-finger-based ATFs specifically recognize targeting sites in chromosomes and effectively up- and downregulate expression of their target genes not only in vitro, but also in vivo. In this review, after briefly introducing Cys2His2-type ZFPs, I will review the studies of endogenous human gene regulation by zinc-finger-based ATFs and other applications as well.

Original languageEnglish
Pages (from-to)513-526
Number of pages14
JournalAdvanced Drug Delivery Reviews
Volume61
Issue number7-8
DOIs
Publication statusPublished - Jul 2 2009
Externally publishedYes

Fingerprint

Zinc Fingers
Transcription Factors
DNA
DNA-Binding Proteins
Genes
Up-Regulation
Down-Regulation
Chromosomes
Gene Expression
Proteins

Keywords

  • Antiviral therapy
  • Artificial transcription factor
  • DNA recognition
  • Gene regulation
  • Gene therapy
  • Transcriptional effector domain
  • Zinc-finger proteins

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Zinc-finger-based artificial transcription factors and their applications. / Sera, Takashi.

In: Advanced Drug Delivery Reviews, Vol. 61, No. 7-8, 02.07.2009, p. 513-526.

Research output: Contribution to journalArticle

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