Abstract
The contaminants in deionized and distilled water (DDI water) boiled with polystyrene resin inhibited A-type monoamine oxidase (MAO, MAO-A preferentially deaminates serotonin and norepinephrine and regulates these amines concentration) activity in monkey brain mitochondria. To identify these contaminants, we attempted measurements by HPLC, FT-IR and NMR. The compound inhibiting MAO-A activity was zinc benzoate. Although it potently inhibited MAO-A activity, zinc benzoate did not effect MAO-B in monkey brain mitochondria. It also reversibly and competitively inhibited MAO-A activity in a dose-dependent manner. Zinc benzoate, however, did not inhibit either MAO-A or -B activities in rat brain mitochondria. These results indicate that zinc benzoate, which inhibits MAO-A activity, is easily incorporated in DDI water by boiling polystyrene and also may be a contaminating environmental chemical compound that alters the levels of serotonin and norepinephrine in the central nervous system.
| Original language | English |
|---|---|
| Pages (from-to) | 161-165 |
| Number of pages | 5 |
| Journal | Toxicology Letters |
| Volume | 145 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Nov 30 2003 |
| Externally published | Yes |
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Keywords
- Environmental compound
- MAO-A
- MAO-B
- Monkey brain mitochondria
- Zinc benzoate
ASJC Scopus subject areas
- Toxicology
Cite this
Zinc benzoate, a contaminating environmental compound derived from polystyrene resin inhibits A-type monoamine oxidase. / Egashira, Toru; Sakai, Kumiko; Takayama, Fusako; Sakurai, Mami; Yoshida, Satoshi.
In: Toxicology Letters, Vol. 145, No. 2, 30.11.2003, p. 161-165.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Zinc benzoate, a contaminating environmental compound derived from polystyrene resin inhibits A-type monoamine oxidase
AU - Egashira, Toru
AU - Sakai, Kumiko
AU - Takayama, Fusako
AU - Sakurai, Mami
AU - Yoshida, Satoshi
PY - 2003/11/30
Y1 - 2003/11/30
N2 - The contaminants in deionized and distilled water (DDI water) boiled with polystyrene resin inhibited A-type monoamine oxidase (MAO, MAO-A preferentially deaminates serotonin and norepinephrine and regulates these amines concentration) activity in monkey brain mitochondria. To identify these contaminants, we attempted measurements by HPLC, FT-IR and NMR. The compound inhibiting MAO-A activity was zinc benzoate. Although it potently inhibited MAO-A activity, zinc benzoate did not effect MAO-B in monkey brain mitochondria. It also reversibly and competitively inhibited MAO-A activity in a dose-dependent manner. Zinc benzoate, however, did not inhibit either MAO-A or -B activities in rat brain mitochondria. These results indicate that zinc benzoate, which inhibits MAO-A activity, is easily incorporated in DDI water by boiling polystyrene and also may be a contaminating environmental chemical compound that alters the levels of serotonin and norepinephrine in the central nervous system.
AB - The contaminants in deionized and distilled water (DDI water) boiled with polystyrene resin inhibited A-type monoamine oxidase (MAO, MAO-A preferentially deaminates serotonin and norepinephrine and regulates these amines concentration) activity in monkey brain mitochondria. To identify these contaminants, we attempted measurements by HPLC, FT-IR and NMR. The compound inhibiting MAO-A activity was zinc benzoate. Although it potently inhibited MAO-A activity, zinc benzoate did not effect MAO-B in monkey brain mitochondria. It also reversibly and competitively inhibited MAO-A activity in a dose-dependent manner. Zinc benzoate, however, did not inhibit either MAO-A or -B activities in rat brain mitochondria. These results indicate that zinc benzoate, which inhibits MAO-A activity, is easily incorporated in DDI water by boiling polystyrene and also may be a contaminating environmental chemical compound that alters the levels of serotonin and norepinephrine in the central nervous system.
KW - Environmental compound
KW - MAO-A
KW - MAO-B
KW - Monkey brain mitochondria
KW - Zinc benzoate
UR - http://www.scopus.com/inward/record.url?scp=0142227668&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0142227668&partnerID=8YFLogxK
U2 - 10.1016/S0378-4274(03)00286-8
DO - 10.1016/S0378-4274(03)00286-8
M3 - Article
C2 - 14581168
AN - SCOPUS:0142227668
VL - 145
SP - 161
EP - 165
JO - Toxicology Letters
JF - Toxicology Letters
SN - 0378-4274
IS - 2
ER -