Z-chromosome specific primers for chicken-quail hybrid blastoderm

Soichi Takagi, Tamao Ono, Akira Tsukada, Yusuke Atsumi, Shusei Mizushima, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.

Original languageEnglish
Pages (from-to)209-212
Number of pages4
JournalJournal of Poultry Science
Volume44
Issue number2
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Chicken
  • PCR
  • PKCI
  • Quail
  • Z-chromosome

ASJC Scopus subject areas

  • Animal Science and Zoology

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