Z-chromosome specific primers for chicken-quail hybrid blastoderm

Soichi Takagi, Tamao Ono, Akira Tsukada, Yusuke Atsumi, Shusei Mizushima, Noboru Saito, Kiyoshi Shimada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.

Original languageEnglish
Pages (from-to)209-212
Number of pages4
JournalJournal of Poultry Science
Volume44
Issue number2
DOIs
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Z chromosome
blastoderm
quails
chickens
polymerase chain reaction
genomics
DNA
oocytes
genes
spermatozoa

Keywords

  • Chicken
  • PCR
  • PKCI
  • Quail
  • Z-chromosome

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Takagi, S., Ono, T., Tsukada, A., Atsumi, Y., Mizushima, S., Saito, N., & Shimada, K. (2007). Z-chromosome specific primers for chicken-quail hybrid blastoderm. Journal of Poultry Science, 44(2), 209-212. https://doi.org/10.2141/jpsa.44.209

Z-chromosome specific primers for chicken-quail hybrid blastoderm. / Takagi, Soichi; Ono, Tamao; Tsukada, Akira; Atsumi, Yusuke; Mizushima, Shusei; Saito, Noboru; Shimada, Kiyoshi.

In: Journal of Poultry Science, Vol. 44, No. 2, 2007, p. 209-212.

Research output: Contribution to journalArticle

Takagi, S, Ono, T, Tsukada, A, Atsumi, Y, Mizushima, S, Saito, N & Shimada, K 2007, 'Z-chromosome specific primers for chicken-quail hybrid blastoderm', Journal of Poultry Science, vol. 44, no. 2, pp. 209-212. https://doi.org/10.2141/jpsa.44.209
Takagi, Soichi ; Ono, Tamao ; Tsukada, Akira ; Atsumi, Yusuke ; Mizushima, Shusei ; Saito, Noboru ; Shimada, Kiyoshi. / Z-chromosome specific primers for chicken-quail hybrid blastoderm. In: Journal of Poultry Science. 2007 ; Vol. 44, No. 2. pp. 209-212.
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