Young adult patients with squamous cell carcinoma of the tongue strongly express p16 without human papillomavirus infection

Tomoyasu Tachibana, Yorihisa Orita, Yuka Gion, Kentaro Miki, Kana Ikegami, Hidenori Marunaka, Takuma Makino, Yusuke Akagi, Naoki Akisada, Munechika Tsumura, Toshihiro Ito, Tadashi Yoshino, Kazunori Nishizaki, Yasuharu Sato

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Long-term smoking and drinking are known to contribute to the onset of tongue cancer (TC). However, the increasing incidence of TC in younger adults has been suggested to be associated with other factors. Objectives: The present study investigated the relationship between TC and human papillomavirus (HPV) infection. Material and methods: Clinical records and surgically resected specimens from 86 patients (<40-years-old, n = 12; ≥40-years-old, n = 74) with TC were analyzed. Strong nuclear and cytoplasmic p16 staining was considered positive. HPV DNA (high-risk subtypes: 16, 18, 31, 33, 35, 52b, and 58; low-risk subtypes: 6 and 11) was detected using consensus primer-mediated polymerase chain reaction. Results: Strong p16 expression was observed in 10 (11.6%) patients. HPV DNA was detected in 9 (10.5%) patients (high-risk subtypes, n = 2; low-risk subtypes, n = 7). Strong p16 expression was observed more frequently among younger adults than among older adults (33.3% vs. 8.1%; p =.045). p16 staining did not correlate with the detection of HPV DNA (correlation coefficient, 0.113; p =.300). Conclusions and significance: In TC, p16 expression was not associated with HPV infection, suggesting that it may be caused by a different mechanism.

Original languageEnglish
JournalActa Oto-Laryngologica
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Tongue Neoplasms
Papillomavirus Infections
Tongue
Young Adult
Squamous Cell Carcinoma
DNA
Staining and Labeling
Drinking
Smoking
Polymerase Chain Reaction
Incidence

Keywords

  • Human papillomavirus
  • p16 protein
  • tongue cancer

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Young adult patients with squamous cell carcinoma of the tongue strongly express p16 without human papillomavirus infection. / Tachibana, Tomoyasu; Orita, Yorihisa; Gion, Yuka; Miki, Kentaro; Ikegami, Kana; Marunaka, Hidenori; Makino, Takuma; Akagi, Yusuke; Akisada, Naoki; Tsumura, Munechika; Ito, Toshihiro; Yoshino, Tadashi; Nishizaki, Kazunori; Sato, Yasuharu.

In: Acta Oto-Laryngologica, 01.01.2019.

Research output: Contribution to journalArticle

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abstract = "Background: Long-term smoking and drinking are known to contribute to the onset of tongue cancer (TC). However, the increasing incidence of TC in younger adults has been suggested to be associated with other factors. Objectives: The present study investigated the relationship between TC and human papillomavirus (HPV) infection. Material and methods: Clinical records and surgically resected specimens from 86 patients (<40-years-old, n = 12; ≥40-years-old, n = 74) with TC were analyzed. Strong nuclear and cytoplasmic p16 staining was considered positive. HPV DNA (high-risk subtypes: 16, 18, 31, 33, 35, 52b, and 58; low-risk subtypes: 6 and 11) was detected using consensus primer-mediated polymerase chain reaction. Results: Strong p16 expression was observed in 10 (11.6{\%}) patients. HPV DNA was detected in 9 (10.5{\%}) patients (high-risk subtypes, n = 2; low-risk subtypes, n = 7). Strong p16 expression was observed more frequently among younger adults than among older adults (33.3{\%} vs. 8.1{\%}; p =.045). p16 staining did not correlate with the detection of HPV DNA (correlation coefficient, 0.113; p =.300). Conclusions and significance: In TC, p16 expression was not associated with HPV infection, suggesting that it may be caused by a different mechanism.",
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AU - Tachibana, Tomoyasu

AU - Orita, Yorihisa

AU - Gion, Yuka

AU - Miki, Kentaro

AU - Ikegami, Kana

AU - Marunaka, Hidenori

AU - Makino, Takuma

AU - Akagi, Yusuke

AU - Akisada, Naoki

AU - Tsumura, Munechika

AU - Ito, Toshihiro

AU - Yoshino, Tadashi

AU - Nishizaki, Kazunori

AU - Sato, Yasuharu

PY - 2019/1/1

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