Yeast elicitor-induced stomatal closure and peroxidase-mediated ros production in Arabidopsis

Md Atiqur Rahman Khokon, Mohammad Anowar Hossain, Shintaro Munemasa, Misugi Uraji, Yoshimasa Nakamura, Izumi Mori, Yoshiyuki Murata

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Yeast elicitor (YEL) induces stomatal closure. We investigated reactive oxygen species (ROS) production, nitric oxide (NO) production and [Ca 2]cyt oscillations to clarify YEL signaling in Arabidopsis guard cells. YEL induced ROS accumulation in guard cells. A peroxidase inhibitor [salicylhydroxamic acid (SHAM)] inhibited the stomatal closure and the ROS accumulation, but neither the atrbohD atrbohF mutation nor an NADPH oxidase inhibitor [diphenylene iodonium chloride (DPI)] had any effect. An NO scavenger [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO)] inhibited the YEL-induced stomatal closure and SHAM abolished NO production. YEL-elicited [Ca2]cyt oscillations were inhibited by SHAM but not by the atrbohD atrbohF mutation. These results indicate that YEL induces stomatal closure accompanied by ROS production mediated by peroxidases and NO production.

Original languageEnglish
Pages (from-to)1915-1921
Number of pages7
JournalPlant and Cell Physiology
Volume51
Issue number11
DOIs
Publication statusPublished - Nov 2010

Fingerprint

Arabidopsis
Peroxidase
peroxidase
Yeasts
yeasts
nitric oxide
reactive oxygen species
Reactive Oxygen Species
Nitric Oxide
guard cells
oscillation
acids
mutation
Peroxidases
Mutation
NADPH Oxidase
peroxidases
oxides
elicitors
Chlorides

Keywords

  • [Ca]cyt oscillations
  • Arabidopsis thaliana
  • NO
  • Peroxidase
  • ROS production
  • Yeast elicitor

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Yeast elicitor-induced stomatal closure and peroxidase-mediated ros production in Arabidopsis. / Khokon, Md Atiqur Rahman; Hossain, Mohammad Anowar; Munemasa, Shintaro; Uraji, Misugi; Nakamura, Yoshimasa; Mori, Izumi; Murata, Yoshiyuki.

In: Plant and Cell Physiology, Vol. 51, No. 11, 11.2010, p. 1915-1921.

Research output: Contribution to journalArticle

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