Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation

Ken Ichi Takemaru, Satoshi Harashima, Hitoshi Ueda, Susumu Hirose

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as a bridging molecule that connects the Drosophila nuclear receptor FTZ-F1 and TATA-binding protein (TBP). The MBF1 sequence is highly conserved across species from Saccharomyces cerevisiae to human. Here we provide evidence acquired in vitro and in vivo that yeast MBF1 mediates GCN4-dependent transcriptional activation by bridging the DNA-binding region of GCN4 and TBP. These findings indicate that the coactivator MBF1 functions by recruiting TBP to promoters where DNA-binding regulators are bound.

Original languageEnglish
Pages (from-to)4971-4976
Number of pages6
JournalMolecular and Cellular Biology
Volume18
Issue number9
Publication statusPublished - 1998
Externally publishedYes

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TATA-Box Binding Protein
Transcriptional Activation
Yeasts
DNA
Cytoplasmic and Nuclear Receptors
Drosophila
Saccharomyces cerevisiae
Transcription Factors
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation. / Takemaru, Ken Ichi; Harashima, Satoshi; Ueda, Hitoshi; Hirose, Susumu.

In: Molecular and Cellular Biology, Vol. 18, No. 9, 1998, p. 4971-4976.

Research output: Contribution to journalArticle

Takemaru, KI, Harashima, S, Ueda, H & Hirose, S 1998, 'Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation', Molecular and Cellular Biology, vol. 18, no. 9, pp. 4971-4976.
Takemaru, Ken Ichi ; Harashima, Satoshi ; Ueda, Hitoshi ; Hirose, Susumu. / Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation. In: Molecular and Cellular Biology. 1998 ; Vol. 18, No. 9. pp. 4971-4976.
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