XTH31, encoding an in vitro XEH/XET-active enzyme, regulates aluminum sensitivity by modulating in vivo XET action, cell wall xyloglucan content, and aluminum binding capacity in arabidopsisw

Shao Jian Zheng, Xiao Fang Zhu, Yuan Zhi Shi, Guijie Lei, Stephen C. Fry, Bao Cai Zhang, Yi Hua Zhou, Janet Braam, Tao Jiang, Xiao Yan Xu, Chuan Zao Mao, Yuan Jiang Pan, Jian Li Yang, Ping Wu

Research output: Contribution to journalArticle

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Abstract

Xyloglucan endohydrolase (XEH) and xyloglucan endotransglucosylase (XET) activities, encoded by xyloglucan endotransglucosylase-hydrolase (XTH) genes, are involved in cell wall extension by cutting or cutting and rejoining xyloglucan chains, respectively. However, the physiological significance of this biochemical activity remains incompletely understood. Here, we find that an XTH31 T-DNA insertion mutant, xth31, is more Al resistant than the wild type. XTH31 is bound to the plasma membrane and the encoding gene is expressed in the root elongation zone and in nascent leaves, suggesting a role in cell expansion. XTH31 transcript accumulation is strongly downregulated by Al treatment. XTH31 expression in yeast yields a protein with an in vitro XEH:XET activity ratio of >5000:1. xth31 accumulates significantly less Al in the root apex and cell wall, shows remarkably lower in vivo XET action and extractable XET activity, has a lower xyloglucan content, and exhibits slower elongation. An exogenous supply of xyloglucan significantly ameliorates Al toxicity by reducing Al accumulation in the roots, owing to the formation of an Al-xyloglucan complex in the medium, as verified by an obvious change in chemical shift of 27Al-NMR. Taken together, the data indicate that XTH31 affects Al sensitivity by modulating cell wall xyloglucan content and Al binding capacity.

Original languageEnglish
Pages (from-to)4731-4747
Number of pages17
JournalPlant Cell
Volume24
Issue number11
DOIs
Publication statusPublished - Nov 1 2012
Externally publishedYes

Fingerprint

xyloglucan - xyloglucosyltransferase
xyloglucan:xyloglucosyl transferase
xyloglucans
binding capacity
Aluminum
Cell Wall
aluminum
cell walls
Enzymes
enzymes
hydrolases
xyloglucan
In Vitro Techniques
Genes
root growth
plasma membrane
Down-Regulation
genes
Yeasts
Cell Membrane

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

XTH31, encoding an in vitro XEH/XET-active enzyme, regulates aluminum sensitivity by modulating in vivo XET action, cell wall xyloglucan content, and aluminum binding capacity in arabidopsisw. / Zheng, Shao Jian; Zhu, Xiao Fang; Shi, Yuan Zhi; Lei, Guijie; Fry, Stephen C.; Zhang, Bao Cai; Zhou, Yi Hua; Braam, Janet; Jiang, Tao; Xu, Xiao Yan; Mao, Chuan Zao; Pan, Yuan Jiang; Yang, Jian Li; Wu, Ping.

In: Plant Cell, Vol. 24, No. 11, 01.11.2012, p. 4731-4747.

Research output: Contribution to journalArticle

Zheng, SJ, Zhu, XF, Shi, YZ, Lei, G, Fry, SC, Zhang, BC, Zhou, YH, Braam, J, Jiang, T, Xu, XY, Mao, CZ, Pan, YJ, Yang, JL & Wu, P 2012, 'XTH31, encoding an in vitro XEH/XET-active enzyme, regulates aluminum sensitivity by modulating in vivo XET action, cell wall xyloglucan content, and aluminum binding capacity in arabidopsisw', Plant Cell, vol. 24, no. 11, pp. 4731-4747. https://doi.org/10.1105/tpc.112.106039
Zheng, Shao Jian ; Zhu, Xiao Fang ; Shi, Yuan Zhi ; Lei, Guijie ; Fry, Stephen C. ; Zhang, Bao Cai ; Zhou, Yi Hua ; Braam, Janet ; Jiang, Tao ; Xu, Xiao Yan ; Mao, Chuan Zao ; Pan, Yuan Jiang ; Yang, Jian Li ; Wu, Ping. / XTH31, encoding an in vitro XEH/XET-active enzyme, regulates aluminum sensitivity by modulating in vivo XET action, cell wall xyloglucan content, and aluminum binding capacity in arabidopsisw. In: Plant Cell. 2012 ; Vol. 24, No. 11. pp. 4731-4747.
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abstract = "Xyloglucan endohydrolase (XEH) and xyloglucan endotransglucosylase (XET) activities, encoded by xyloglucan endotransglucosylase-hydrolase (XTH) genes, are involved in cell wall extension by cutting or cutting and rejoining xyloglucan chains, respectively. However, the physiological significance of this biochemical activity remains incompletely understood. Here, we find that an XTH31 T-DNA insertion mutant, xth31, is more Al resistant than the wild type. XTH31 is bound to the plasma membrane and the encoding gene is expressed in the root elongation zone and in nascent leaves, suggesting a role in cell expansion. XTH31 transcript accumulation is strongly downregulated by Al treatment. XTH31 expression in yeast yields a protein with an in vitro XEH:XET activity ratio of >5000:1. xth31 accumulates significantly less Al in the root apex and cell wall, shows remarkably lower in vivo XET action and extractable XET activity, has a lower xyloglucan content, and exhibits slower elongation. An exogenous supply of xyloglucan significantly ameliorates Al toxicity by reducing Al accumulation in the roots, owing to the formation of an Al-xyloglucan complex in the medium, as verified by an obvious change in chemical shift of 27Al-NMR. Taken together, the data indicate that XTH31 affects Al sensitivity by modulating cell wall xyloglucan content and Al binding capacity.",
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AU - Zheng, Shao Jian

AU - Zhu, Xiao Fang

AU - Shi, Yuan Zhi

AU - Lei, Guijie

AU - Fry, Stephen C.

AU - Zhang, Bao Cai

AU - Zhou, Yi Hua

AU - Braam, Janet

AU - Jiang, Tao

AU - Xu, Xiao Yan

AU - Mao, Chuan Zao

AU - Pan, Yuan Jiang

AU - Yang, Jian Li

AU - Wu, Ping

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