Xenogeneic dopaminergic grafts reverse behavioral deficits induced by 6-OHDA in rodents: Effect of 15-deoxyspergualin treatment

Jiawei Zhou; Isao Date; Kyoji Sakai; Yusuke Yoshimoto; Tomohisa Furuta; Shoji Asari; Takashi Ohmoto

doi:10.1016/0304-3940(93)90234-C

Xenogeneic dopaminergic grafts reverse behavioral deficits induced by 6-OHDA in rodents: Effect of 15-deoxyspergualin treatment

Jiawei Zhou, Isao Date, Kyoji Sakai, Yusuke Yoshimoto, Tomohisa Furuta, Shoji Asari, Takashi Ohmoto

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Foetal rat mesencephalic dopamine-containing tissue was transplanted into the lateral ventricle of mice previously subjected to a 6-OHDA lesion of dopaminergic nerve terminals in the corpus striatum. The graft recipients were immunosuppressed by subcutaneous injections of 15-deoxy-spergualin (DSG). Four weeks postgrafting, all DSG-treated mice showed partial or complete functional compensation in amphetamine-induced motor asymmetry. The immunohistochemical staining of tyrosine hydroxylase (TH) revealed large numbers of surviving dopamine neurons and abundant fibers in the grafted animals. In contrast, all grafts in non-DSG-treated animals were rejected and functional compensation was lacking. It is concluded that DSG treatment promotes xenogeneic intracerebral graft survival, recovery of function and reduce the histological sign of rejection.

Original language	English
Pages (from-to)	81-84
Number of pages	4
Journal	Neuroscience Letters
Volume	163
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(93)90234-C
Publication status	Published - Nov 26 1993

Keywords

15-Deoxyspergualin
Dopamine
Immunosuppressant
Neural transplantation
Parkinson's disease
Rodent

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0304-3940(93)90234-C

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@article{34083b7cf1df48209cca1ef29f417eec,

title = "Xenogeneic dopaminergic grafts reverse behavioral deficits induced by 6-OHDA in rodents: Effect of 15-deoxyspergualin treatment",

abstract = "Foetal rat mesencephalic dopamine-containing tissue was transplanted into the lateral ventricle of mice previously subjected to a 6-OHDA lesion of dopaminergic nerve terminals in the corpus striatum. The graft recipients were immunosuppressed by subcutaneous injections of 15-deoxy-spergualin (DSG). Four weeks postgrafting, all DSG-treated mice showed partial or complete functional compensation in amphetamine-induced motor asymmetry. The immunohistochemical staining of tyrosine hydroxylase (TH) revealed large numbers of surviving dopamine neurons and abundant fibers in the grafted animals. In contrast, all grafts in non-DSG-treated animals were rejected and functional compensation was lacking. It is concluded that DSG treatment promotes xenogeneic intracerebral graft survival, recovery of function and reduce the histological sign of rejection.",

keywords = "15-Deoxyspergualin, Dopamine, Immunosuppressant, Neural transplantation, Parkinson's disease, Rodent",

author = "Jiawei Zhou and Isao Date and Kyoji Sakai and Yusuke Yoshimoto and Tomohisa Furuta and Shoji Asari and Takashi Ohmoto",

note = "Funding Information: script. This work was supported in part by grant from the Ministry of Education, Science and Culture, Japan, 04770989 to I.D.",

year = "1993",

month = nov,

day = "26",

doi = "10.1016/0304-3940(93)90234-C",

language = "English",

volume = "163",

pages = "81--84",

journal = "Neuroscience Letters",

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T1 - Xenogeneic dopaminergic grafts reverse behavioral deficits induced by 6-OHDA in rodents

T2 - Effect of 15-deoxyspergualin treatment

AU - Zhou, Jiawei

AU - Date, Isao

AU - Sakai, Kyoji

AU - Yoshimoto, Yusuke

AU - Furuta, Tomohisa

AU - Asari, Shoji

AU - Ohmoto, Takashi

N1 - Funding Information: script. This work was supported in part by grant from the Ministry of Education, Science and Culture, Japan, 04770989 to I.D.

PY - 1993/11/26

Y1 - 1993/11/26

N2 - Foetal rat mesencephalic dopamine-containing tissue was transplanted into the lateral ventricle of mice previously subjected to a 6-OHDA lesion of dopaminergic nerve terminals in the corpus striatum. The graft recipients were immunosuppressed by subcutaneous injections of 15-deoxy-spergualin (DSG). Four weeks postgrafting, all DSG-treated mice showed partial or complete functional compensation in amphetamine-induced motor asymmetry. The immunohistochemical staining of tyrosine hydroxylase (TH) revealed large numbers of surviving dopamine neurons and abundant fibers in the grafted animals. In contrast, all grafts in non-DSG-treated animals were rejected and functional compensation was lacking. It is concluded that DSG treatment promotes xenogeneic intracerebral graft survival, recovery of function and reduce the histological sign of rejection.

AB - Foetal rat mesencephalic dopamine-containing tissue was transplanted into the lateral ventricle of mice previously subjected to a 6-OHDA lesion of dopaminergic nerve terminals in the corpus striatum. The graft recipients were immunosuppressed by subcutaneous injections of 15-deoxy-spergualin (DSG). Four weeks postgrafting, all DSG-treated mice showed partial or complete functional compensation in amphetamine-induced motor asymmetry. The immunohistochemical staining of tyrosine hydroxylase (TH) revealed large numbers of surviving dopamine neurons and abundant fibers in the grafted animals. In contrast, all grafts in non-DSG-treated animals were rejected and functional compensation was lacking. It is concluded that DSG treatment promotes xenogeneic intracerebral graft survival, recovery of function and reduce the histological sign of rejection.

KW - 15-Deoxyspergualin

KW - Dopamine

KW - Immunosuppressant

KW - Neural transplantation

KW - Parkinson's disease

KW - Rodent

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Xenogeneic dopaminergic grafts reverse behavioral deficits induced by 6-OHDA in rodents: Effect of 15-deoxyspergualin treatment

Abstract

Keywords

ASJC Scopus subject areas

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