Xanthene dyes induce membrane permeabilization of bacteria and erythrocytes by photoinactivation

Hisato Kato, Keiko Komagoe, Yuka Nakanishi, Tsuyoshi Inoue, Takashi Katsu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We analyzed the photoinactivation of the membrane functions of bacteria and erythrocytes induced by xanthene dyes. The dyes tested were rose bengal, phloxine B, erythrosine B and eosin B. These dyes induced the leakage of K + from Staphylococcus aureus cells within minutes of photoirradiation, in the order of rose bengal > phloxine B > erythrosine B > eosin B. The ability of dyes to inhibit respiration was weak, except for rose bengal, and the dyes dissipated the membrane potential in similar time traces with changes in K + permeability. The xanthene dyes also induced the leakage of K + from bovine erythrocytes upon photoirradiation in the same order as that observed with bacteria. Furthermore, we found that the ability to cause the leakage of K + from erythrocytes was associated with dye-induced morphological changes, forming a crenated form from the normal discoid. These results are discussed in connection with the ability of xanthene dyes to generate singlet oxygen and bind to bacterial cells, and further compared with the actions of cationic porphyrins, which induced photoinactivation of bacteria through respiratory inhibition.

Original languageEnglish
Pages (from-to)423-431
Number of pages9
JournalPhotochemistry and Photobiology
Volume88
Issue number2
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Xanthenes
erythrocytes
bacteria
Eosine I Bluish
Bacteria
Coloring Agents
Erythrocytes
dyes
membranes
Membranes
Rose Bengal
Erythrosine
Rose Bengal B
leakage
Singlet Oxygen
staphylococcus
Porphyrins
respiration
cells
porphyrins

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

Xanthene dyes induce membrane permeabilization of bacteria and erythrocytes by photoinactivation. / Kato, Hisato; Komagoe, Keiko; Nakanishi, Yuka; Inoue, Tsuyoshi; Katsu, Takashi.

In: Photochemistry and Photobiology, Vol. 88, No. 2, 03.2012, p. 423-431.

Research output: Contribution to journalArticle

Kato, Hisato ; Komagoe, Keiko ; Nakanishi, Yuka ; Inoue, Tsuyoshi ; Katsu, Takashi. / Xanthene dyes induce membrane permeabilization of bacteria and erythrocytes by photoinactivation. In: Photochemistry and Photobiology. 2012 ; Vol. 88, No. 2. pp. 423-431.
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