X-ray Diffraction from a Left Ventricular Wall of Rat Heart

Naoto Yagi, Juichiro Shimizu, Satoshi Mohri, Jun'Ichi Araki, Kazufumi Nakamura, Hiroshi Okuyama, Hiroko Toyota, Taro Morimoto, Yuki Morizane, Mie Kurusu, Tatsushi Miura, Katsushi Hashimoto, Katsuhiko Tsujioka, Hiroyuki Suga, Fumihiko Kajiya

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24 Citations (Scopus)

Abstract

We studied x-ray diffraction from the left ventricular wall of an excised, perfused whole heart of a rat using x rays from the third-generation synchrotron radiation facility, SPring-8. With the beam at right angles to the long axis of the left ventricle, well-oriented, strong equatorial reflections were observed from the epicardium surface. The reflections became vertically split arcs when the beam passed through myocardium deeper in the wall, and rings were observed when the beam passed into the inner myocardium of the wall. These diffraction patterns were explained by employing a layered-spiral model of the arrangement of muscle fibers in the heart. In a quiescent heart with an expanded left ventricle, the muscle fibers at the epicardium surface were found to have a (1,0) lattice spacing smaller than in the rest of the wall. The intensity ratio of the (1,0) and (1,1) equatorial reflections decreased on contraction with a similar time course in all parts of the wall. The results show that it is possible to assign the origin of reflections in a diffraction diagram from a whole heart. This study offers a basis for interpretation of x-ray diffraction from a beating heart under physiologically and pathologically different conditions.

Original languageEnglish
Pages (from-to)2286-2294
Number of pages9
JournalBiophysical Journal
Volume86
Issue number4
DOIs
Publication statusPublished - Apr 2004

ASJC Scopus subject areas

  • Biophysics

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    Yagi, N., Shimizu, J., Mohri, S., Araki, JI., Nakamura, K., Okuyama, H., Toyota, H., Morimoto, T., Morizane, Y., Kurusu, M., Miura, T., Hashimoto, K., Tsujioka, K., Suga, H., & Kajiya, F. (2004). X-ray Diffraction from a Left Ventricular Wall of Rat Heart. Biophysical Journal, 86(4), 2286-2294. https://doi.org/10.1016/S0006-3495(04)74286-1