X-ray crystallographic structure of RNase Po1 that exhibits antitumor activity

Hiroko Kobayashi, Takuya Katsutani, Yumiko Hara, Naomi Motoyoshi, Tadashi Itagaki, Fusamichi Akita, Akifumi Higashiura, Yusuke Yamada, Norio Inokuchi, Mamoru Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

RNase Po1 is a guanylic acid-specific ribonuclease member of the RNase T1 family from Pleurotus ostreatus. We previously reported that RNase Po1 inhibits the proliferation of human tumor cells, yet RNase T1 and other T1 family RNases are non-toxic. We determined the three-dimensional X-ray structure of RNase Po1 and compared it with that of RNase T1. The catalytic sites are conserved. However, there are three disulfide bonds, one more than in RNase T1. One of the additional disulfide bond is in the catalytic and binding site of RNase Po1, and makes RNase Po1 more stable than RNase T1. A comparison of the electrostatic potential of the molecular surfaces of these two proteins shows that RNase T1 is anionic whereas RNase Po1 is cationic, so RNase Po1 might bind to the plasma membrane electrostatically. We suggest that the structural stability and cationic character of RNase Po1 are critical to the anti-cancer properties of the protein.

Original languageEnglish
Pages (from-to)968-978
Number of pages11
JournalBiological and Pharmaceutical Bulletin
Volume37
Issue number6
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Ribonuclease T1
X-Rays
Disulfides
Catalytic Domain
Guanosine Monophosphate
Pleurotus
ribonuclease Po1
Ribonucleases
Static Electricity
Neoplasms
Membrane Proteins
Binding Sites
Cell Membrane

Keywords

  • Anti-tumor activity
  • Crystal structure
  • Pleurotus ostreatus
  • Ribonuclease

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology
  • Medicine(all)

Cite this

Kobayashi, H., Katsutani, T., Hara, Y., Motoyoshi, N., Itagaki, T., Akita, F., ... Suzuki, M. (2014). X-ray crystallographic structure of RNase Po1 that exhibits antitumor activity. Biological and Pharmaceutical Bulletin, 37(6), 968-978. https://doi.org/10.1248/bpb.b13-00929

X-ray crystallographic structure of RNase Po1 that exhibits antitumor activity. / Kobayashi, Hiroko; Katsutani, Takuya; Hara, Yumiko; Motoyoshi, Naomi; Itagaki, Tadashi; Akita, Fusamichi; Higashiura, Akifumi; Yamada, Yusuke; Inokuchi, Norio; Suzuki, Mamoru.

In: Biological and Pharmaceutical Bulletin, Vol. 37, No. 6, 2014, p. 968-978.

Research output: Contribution to journalArticle

Kobayashi, H, Katsutani, T, Hara, Y, Motoyoshi, N, Itagaki, T, Akita, F, Higashiura, A, Yamada, Y, Inokuchi, N & Suzuki, M 2014, 'X-ray crystallographic structure of RNase Po1 that exhibits antitumor activity', Biological and Pharmaceutical Bulletin, vol. 37, no. 6, pp. 968-978. https://doi.org/10.1248/bpb.b13-00929
Kobayashi, Hiroko ; Katsutani, Takuya ; Hara, Yumiko ; Motoyoshi, Naomi ; Itagaki, Tadashi ; Akita, Fusamichi ; Higashiura, Akifumi ; Yamada, Yusuke ; Inokuchi, Norio ; Suzuki, Mamoru. / X-ray crystallographic structure of RNase Po1 that exhibits antitumor activity. In: Biological and Pharmaceutical Bulletin. 2014 ; Vol. 37, No. 6. pp. 968-978.
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