X-ray crystallographic and biochemical characterizations of a mutant photosystem II complex from Thermosynechococcus vulcanus with the psbTc gene inactivated by an insertion mutation

Takahiro Henmi, Masako Iwai, Masahiko Ikeuchi, Keisuke Kawakami, Jian Ren Shen, Nobuo Kamiya

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The crystal structure of a photosystem II (PSII) dimer from Thermosynechococcus vulcanus with its psbTc gene inactivated by insertion mutation of an antibiotic cassette in a site in the C-terminal region was analyzed at 3.8 Å resolution. In the crystal structure of the mutant PSII, the transmembrane helix of PsbTc remains, whereas the C-terminal loop of PsbTc has disappeared. In addition, the PsbM subunit, which seemed to be lost in a PsbTc-deletion mutant PSII of T. elongatus, is still present. The deletion of the C-terminal loop of PsbTc in the mutant PSII was verified by mass spectrometry. Thus, the insertion mutation of psbTc eliminated only the C-terminal loop of this subunit. Nevertheless, some features of the mutant PSII, namely a destabilization of the dimeric form and a slight decrease of the oxygen-evolving activity, were observed in the mutant, indicating that the C-terminal loop of PsbTc functions to maintain the stability of the PSII dimer and the activity of oxygen evolution.

Original languageEnglish
Pages (from-to)304-307
Number of pages4
JournalJournal of Synchrotron Radiation
Volume15
Issue number3
DOIs
Publication statusPublished - Apr 18 2008

Keywords

  • Mutant
  • Oxygen evolution
  • Photosystem II
  • PsbTc
  • Thermosynechococcus vulcanus

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

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