X-ray crystallographic and biochemical characterizations of a mutant photosystem II complex from Thermosynechococcus vulcanus with the psbTc gene inactivated by an insertion mutation

Takahiro Henmi, Masako Iwai, Masahiko Ikeuchi, Keisuke Kawakami, Jian-Ren Shen, Nobuo Kamiya

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The crystal structure of a photosystem II (PSII) dimer from Thermosynechococcus vulcanus with its psbTc gene inactivated by insertion mutation of an antibiotic cassette in a site in the C-terminal region was analyzed at 3.8 Å resolution. In the crystal structure of the mutant PSII, the transmembrane helix of PsbTc remains, whereas the C-terminal loop of PsbTc has disappeared. In addition, the PsbM subunit, which seemed to be lost in a PsbTc-deletion mutant PSII of T. elongatus, is still present. The deletion of the C-terminal loop of PsbTc in the mutant PSII was verified by mass spectrometry. Thus, the insertion mutation of psbTc eliminated only the C-terminal loop of this subunit. Nevertheless, some features of the mutant PSII, namely a destabilization of the dimeric form and a slight decrease of the oxygen-evolving activity, were observed in the mutant, indicating that the C-terminal loop of PsbTc functions to maintain the stability of the PSII dimer and the activity of oxygen evolution.

Original languageEnglish
Pages (from-to)304-307
Number of pages4
JournalJournal of Synchrotron Radiation
Volume15
Issue number3
DOIs
Publication statusPublished - Apr 18 2008

Fingerprint

mutations
genes
Dimers
insertion
Genes
Crystal structure
X rays
deletion
Oxygen
Antibiotics
Mass spectrometry
x rays
dimers
crystal structure
antibiotics
destabilization
oxygen
helices
mass spectroscopy

Keywords

  • Mutant
  • Oxygen evolution
  • Photosystem II
  • PsbTc
  • Thermosynechococcus vulcanus

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics
  • Radiation
  • Physics and Astronomy (miscellaneous)
  • Nuclear and High Energy Physics

Cite this

X-ray crystallographic and biochemical characterizations of a mutant photosystem II complex from Thermosynechococcus vulcanus with the psbTc gene inactivated by an insertion mutation. / Henmi, Takahiro; Iwai, Masako; Ikeuchi, Masahiko; Kawakami, Keisuke; Shen, Jian-Ren; Kamiya, Nobuo.

In: Journal of Synchrotron Radiation, Vol. 15, No. 3, 18.04.2008, p. 304-307.

Research output: Contribution to journalArticle

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