Wingless-type protein-1 (Wnt-1) expression in primary conventional and unicystic ameloblastomas and their recurrent tumors

Kee Seng Chuah, Chong Huat Siar, Keisuke Nakano, Hitoshi Nagatsuka, Suan Phaik Khoo, Kok Han Ng, Toshiyuki Kawakami

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Wingless-type protein (Wnt) is a family of 19 secreted glycoproteins that function as signaling transducers for cell-cell interaction, cell growth and differentiation. Wnt-1, a highly transforming member, has been implicated in tumorigenesis. The aim of this study was to elucidate the role of this cell signaling molecule in the development and progression of primary ameloblastoma and their recurrent tumors. Wnt-1 expression patterns were examined immunohistochemically in 22 primary and 14 recurrent ameloblastomas. These collectively consisted of the following subtypes: conventional (CA) (n=22), desmoplastic (DA) (n=2) and unicystic (UA) (n=12) ameloblastoma. Results demonstrated that CA (n=20/22; 90.9%), DA (n =2/2; 100%) and UA (11/12; 91.7%) showed high Wnt-1 expression percentages. Strong staining intensity for Wnt-1 was observed more frequently in primary CA (n =10/13; 76.9%) than in their recurrent counterparts (n= 2/9; 22.2%) (p0.05). Keratinizing cells in areas of squamous metaplasia also expressed Wnt-1 more intensely compared to their surrounding polyhedral stellate reticulum-like cells. Tumor islands containing granular cells were also Wnt-1 positive. Present findings confirmed that the Wnt signaling pathway is activated in ameloblastoma. Strong Wnt-1 expression in primary conventional and recurrent unicystic ameloblastoma suggests that Wnt-1 plays a more critical role in these subtypes. Positive expression of Wnt-1 in keratinizing ameloblastomatous tumor epithelium and granular cells complies with the anti-apoptotic properties of Wnt-1. Negative reactivity for Wnt-1 in 3 cases of ameloblastoma suggests that the development and progression of ameloblastoma may occur independent of this cell signaling molecule.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalJournal of Hard Tissue Biology
Volume18
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

Wnt Proteins
Ameloblastoma
Tumors
Proteins
Neoplasms
Cell signaling
Granular Cell Tumor
Reticulum
Glycoproteins
Molecules
Metaplasia
Cell growth
Transducers
Islands
Cell Communication

Keywords

  • Ameloblastoma
  • Immunohistochemistry
  • Wnt-1

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Wingless-type protein-1 (Wnt-1) expression in primary conventional and unicystic ameloblastomas and their recurrent tumors. / Chuah, Kee Seng; Siar, Chong Huat; Nakano, Keisuke; Nagatsuka, Hitoshi; Khoo, Suan Phaik; Ng, Kok Han; Kawakami, Toshiyuki.

In: Journal of Hard Tissue Biology, Vol. 18, No. 2, 2009, p. 63-70.

Research output: Contribution to journalArticle

Chuah, Kee Seng ; Siar, Chong Huat ; Nakano, Keisuke ; Nagatsuka, Hitoshi ; Khoo, Suan Phaik ; Ng, Kok Han ; Kawakami, Toshiyuki. / Wingless-type protein-1 (Wnt-1) expression in primary conventional and unicystic ameloblastomas and their recurrent tumors. In: Journal of Hard Tissue Biology. 2009 ; Vol. 18, No. 2. pp. 63-70.
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