Whole blood interferon-gamma assay for baseline tuberculosis screening among Japanese healthcare students

Katsuyuki Hotta, Toshio Ogura, Kenji Nishii, Tsuyoshi Kodani, Masaru Onishi, Yukito Shimizu, Arihiko Kanehiro, Katsuyuki Kiura, Mitsune Tanimoto, Kazuo Tobe

Research output: Contribution to journalArticle

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Abstract

Background. The whole blood interferon-gamma assay (OuantIPER0N-TB-2G:QFT) has not been fully evaluated as a baseline tuberculosis screening test in Japanese healthcare students commencing clinical contact. The aim of this study was to compare the results from the QFT with those from the tuberculin skin test (TST) in a population deemed to be at a low risk for infection with Mycobacterium tuberculosis. Methodology/Principal Findigs. Healthcare students recruited at Okayama University received both the TST and the QFT to assess the level of agreement between these two tests. The interleukin-10 levels before and after exposure to M tuberculosis-specific antigens {early-secreted antigenic target 6-kDa protein [ESAT-6] and culture filtrate protein 10 [CFP-10]) were also measured. Of the S36 healthcare students, most of whom have been vaccinated with bacillus-Calmette-Guérin (BCG), 207 MOM were enrolled in this, study. The agreement between the QFT and the TST results was poor, with positive result rates of 1.4% vs. 27,5%, respectively. A multivariate analysis also revealed that the induration diameter of the TST was not affected by the interferon-gamma concentration after exposure to either of the antigens but was influenced by the number of BCG needle scars (p=0.046). The whole blood interieukin-10 assay revealed that after antigen exposure, the median increases in Interknukln-10 concentration was higher in the subgroup with the small increase in interferon-gamma concentration than in the subgroup with the large increase in interferon-gamma concentration (0.3 vs. 0 pg/ml; p=0.004). Conclusions/Significance. As a baseline screening test for low-risk Japanese healthcare students at their course entry, QFT yielded quite discordant results, compared with the TST, probably because of the low specificity of the TST results in the BCG-vaccinated population. We also found, for the first time, that the change in the interieukin-10 level after exposure to specific antigens was inversely associated with that in the interferon-gamma level in a low-risk population.

Original languageEnglish
Article numbere803
JournalPLoS One
Volume2
Issue number8
DOIs
Publication statusPublished - Aug 29 2007

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Tuberculin Test
tuberculin
skin tests
Tuberculin
Skin Tests
interferon-gamma
tuberculosis
health services
Interferon-gamma
Assays
Skin
Screening
students
Tuberculosis
Blood
Students
screening
Delivery of Health Care
blood
assays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Whole blood interferon-gamma assay for baseline tuberculosis screening among Japanese healthcare students. / Hotta, Katsuyuki; Ogura, Toshio; Nishii, Kenji; Kodani, Tsuyoshi; Onishi, Masaru; Shimizu, Yukito; Kanehiro, Arihiko; Kiura, Katsuyuki; Tanimoto, Mitsune; Tobe, Kazuo.

In: PLoS One, Vol. 2, No. 8, e803, 29.08.2007.

Research output: Contribution to journalArticle

Hotta, Katsuyuki ; Ogura, Toshio ; Nishii, Kenji ; Kodani, Tsuyoshi ; Onishi, Masaru ; Shimizu, Yukito ; Kanehiro, Arihiko ; Kiura, Katsuyuki ; Tanimoto, Mitsune ; Tobe, Kazuo. / Whole blood interferon-gamma assay for baseline tuberculosis screening among Japanese healthcare students. In: PLoS One. 2007 ; Vol. 2, No. 8.
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abstract = "Background. The whole blood interferon-gamma assay (OuantIPER0N-TB-2G:QFT) has not been fully evaluated as a baseline tuberculosis screening test in Japanese healthcare students commencing clinical contact. The aim of this study was to compare the results from the QFT with those from the tuberculin skin test (TST) in a population deemed to be at a low risk for infection with Mycobacterium tuberculosis. Methodology/Principal Findigs. Healthcare students recruited at Okayama University received both the TST and the QFT to assess the level of agreement between these two tests. The interleukin-10 levels before and after exposure to M tuberculosis-specific antigens {early-secreted antigenic target 6-kDa protein [ESAT-6] and culture filtrate protein 10 [CFP-10]) were also measured. Of the S36 healthcare students, most of whom have been vaccinated with bacillus-Calmette-Gu{\'e}rin (BCG), 207 MOM were enrolled in this, study. The agreement between the QFT and the TST results was poor, with positive result rates of 1.4{\%} vs. 27,5{\%}, respectively. A multivariate analysis also revealed that the induration diameter of the TST was not affected by the interferon-gamma concentration after exposure to either of the antigens but was influenced by the number of BCG needle scars (p=0.046). The whole blood interieukin-10 assay revealed that after antigen exposure, the median increases in Interknukln-10 concentration was higher in the subgroup with the small increase in interferon-gamma concentration than in the subgroup with the large increase in interferon-gamma concentration (0.3 vs. 0 pg/ml; p=0.004). Conclusions/Significance. As a baseline screening test for low-risk Japanese healthcare students at their course entry, QFT yielded quite discordant results, compared with the TST, probably because of the low specificity of the TST results in the BCG-vaccinated population. We also found, for the first time, that the change in the interieukin-10 level after exposure to specific antigens was inversely associated with that in the interferon-gamma level in a low-risk population.",
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AU - Ogura, Toshio

AU - Nishii, Kenji

AU - Kodani, Tsuyoshi

AU - Onishi, Masaru

AU - Shimizu, Yukito

AU - Kanehiro, Arihiko

AU - Kiura, Katsuyuki

AU - Tanimoto, Mitsune

AU - Tobe, Kazuo

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N2 - Background. The whole blood interferon-gamma assay (OuantIPER0N-TB-2G:QFT) has not been fully evaluated as a baseline tuberculosis screening test in Japanese healthcare students commencing clinical contact. The aim of this study was to compare the results from the QFT with those from the tuberculin skin test (TST) in a population deemed to be at a low risk for infection with Mycobacterium tuberculosis. Methodology/Principal Findigs. Healthcare students recruited at Okayama University received both the TST and the QFT to assess the level of agreement between these two tests. The interleukin-10 levels before and after exposure to M tuberculosis-specific antigens {early-secreted antigenic target 6-kDa protein [ESAT-6] and culture filtrate protein 10 [CFP-10]) were also measured. Of the S36 healthcare students, most of whom have been vaccinated with bacillus-Calmette-Guérin (BCG), 207 MOM were enrolled in this, study. The agreement between the QFT and the TST results was poor, with positive result rates of 1.4% vs. 27,5%, respectively. A multivariate analysis also revealed that the induration diameter of the TST was not affected by the interferon-gamma concentration after exposure to either of the antigens but was influenced by the number of BCG needle scars (p=0.046). The whole blood interieukin-10 assay revealed that after antigen exposure, the median increases in Interknukln-10 concentration was higher in the subgroup with the small increase in interferon-gamma concentration than in the subgroup with the large increase in interferon-gamma concentration (0.3 vs. 0 pg/ml; p=0.004). Conclusions/Significance. As a baseline screening test for low-risk Japanese healthcare students at their course entry, QFT yielded quite discordant results, compared with the TST, probably because of the low specificity of the TST results in the BCG-vaccinated population. We also found, for the first time, that the change in the interieukin-10 level after exposure to specific antigens was inversely associated with that in the interferon-gamma level in a low-risk population.

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