Abstract
Western blotting is widely used for protein analysis. We routinely perform such analysis for evaluating the production levels of CCN family proteins in a variety of cells under various conditions. In this chapter, we describe our Western blotting protocol to estimate protein production profiles of CCN family members after having assessed the specificity of the antibodies against each CCN member protein to ensure no crossreaction with other CCN member proteins.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 43-51 |
| Number of pages | 9 |
| Volume | 1489 |
| DOIs | |
| Publication status | Published - 2017 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1489 |
| ISSN (Print) | 10643745 |
Keywords
- Amino acid homology
- Conserved module
- Cross-reaction
- Member-specific antibody
- Redundancy of CCN members
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Kawaki, H., Kubota, S., & Takigawa, M. (2017). Western blotting analysis of CCN proteins in calcified tissues. In Methods in Molecular Biology (Vol. 1489, pp. 43-51). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_5