Western blotting analysis of CCN proteins in calcified tissues

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1 Citation (Scopus)

Abstract

Western blotting is widely used for protein analysis. We routinely perform such analysis for evaluating the production levels of CCN family proteins in a variety of cells under various conditions. In this chapter, we describe our Western blotting protocol to estimate protein production profiles of CCN family members after having assessed the specificity of the antibodies against each CCN member protein to ensure no crossreaction with other CCN member proteins.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages43-51
Number of pages9
Volume1489
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1489
ISSN (Print)10643745

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Keywords

  • Amino acid homology
  • Conserved module
  • Cross-reaction
  • Member-specific antibody
  • Redundancy of CCN members

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kawaki, H., Kubota, S., & Takigawa, M. (2017). Western blotting analysis of CCN proteins in calcified tissues. In Methods in Molecular Biology (Vol. 1489, pp. 43-51). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_5