@inbook{b5e22ab8b3114ec28b93b3d810e0db85,
title = "Western blotting analysis of CCN proteins in calcified tissues",
abstract = "Western blotting is widely used for protein analysis. We routinely perform such analysis for evaluating the production levels of CCN family proteins in a variety of cells under various conditions. In this chapter, we describe our Western blotting protocol to estimate protein production profiles of CCN family members after having assessed the specificity of the antibodies against each CCN member protein to ensure no crossreaction with other CCN member proteins.",
keywords = "Amino acid homology, Conserved module, Cross-reaction, Member-specific antibody, Redundancy of CCN members",
author = "Harumi Kawaki and Satoshi Kubota and Masaharu Takigawa",
note = "Funding Information: This work was supported by JSPS KAKENHI Grant Numbers JP24390415 and JP15H05014 to M.T., JP25462886 to S.K., JP26462984 to H.K., and The Science Research Promotion Fund of The Promotion and Mutual Aid Corporation for Private Schools of Japan to H.K. The authors gratefully thank Ms. Yoshiko Miyake for her secretary assistance.",
year = "2017",
doi = "10.1007/978-1-4939-6430-7_5",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "43--51",
booktitle = "Methods in Molecular Biology",
}