Western blotting analysis of CCN proteins in calcified tissues

Harumi Kawaki; Satoshi Kubota; Masaharu Takigawa

doi:10.1007/978-1-4939-6430-7_5

Western blotting analysis of CCN proteins in calcified tissues

Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

Western blotting is widely used for protein analysis. We routinely perform such analysis for evaluating the production levels of CCN family proteins in a variety of cells under various conditions. In this chapter, we describe our Western blotting protocol to estimate protein production profiles of CCN family members after having assessed the specificity of the antibodies against each CCN member protein to ensure no crossreaction with other CCN member proteins.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	43-51
Number of pages	9
Volume	1489
DOIs	https://doi.org/10.1007/978-1-4939-6430-7_5
Publication status	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1489
ISSN (Print)	10643745

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Keywords

Amino acid homology
Conserved module
Cross-reaction
Member-specific antibody
Redundancy of CCN members

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Kawaki, H., Kubota, S., & Takigawa, M. (2017). Western blotting analysis of CCN proteins in calcified tissues. In Methods in Molecular Biology (Vol. 1489, pp. 43-51). (Methods in Molecular Biology; Vol. 1489). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6430-7_5

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KW - Cross-reaction

KW - Member-specific antibody

KW - Redundancy of CCN members

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Access to Document

10.1007/978-1-4939-6430-7_5